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dc.contributor.authorAlemayehu, Debebe
dc.contributor.authorHannon, John A.
dc.contributor.authorMcAuliffe, Olivia
dc.contributor.authorRoss, R Paul
dc.date.accessioned2020-08-06T15:08:46Z
dc.date.available2020-08-06T15:08:46Z
dc.date.issued2014-02-17
dc.identifier.citationAlemayehu, D., Hannon, J., McAuliffe, O., Ross, R. Characterization of plant-derived lactococci on the basis of their volatile compounds profile when grown in milk. International Journal of Food Microbiology, 2014, 172, 57-61. doi: https://doi.org/10.1016/j.ijfoodmicro.2013.11.024en_US
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/11019/2274
dc.descriptionpeer-revieweden_US
dc.description.abstractA total of twelve strains of lactococci were isolated from grass and vegetables (baby corn and fresh green peas). Ten of the isolates were classified as Lactococcus lactis subsp. lactis and two as Lactococcus lactis subsp. cremoris based on 16S rDNA sequencing. Most of the plant-derived strains were capable of metabolising a wide range of carbohydrates in that they fermented D-mannitol, amygdalin, potassium gluconate, l-arabinose, d-xylose, sucrose and gentibiose. None of the dairy control strains (i.e. L. lactis subsp. cremoris HP, L. lactis subsp. lactis IL1403 and Lactococcus lactis 303) were able to utilize any of these carbohydrates. The technological potential of the isolates as flavour-producing lactococci was evaluated by analysing their growth in milk and their ability to produce volatile compounds using solid phase micro-extraction of the headspace coupled to gas chromatography–mass spectrometry (SPME GC–MS). Principal component analysis (PCA) of the volatile compounds clearly separated the dairy strains from the plant derived strains, with higher levels of most flavour rich compounds. The flavour compounds produced by the plant isolates among others included; fatty acids such as 2- and 3-methylbutanoic acids, and hexanoic acid, several esters (e.g. butyl acetate and ethyl butanoate) and ketones (e.g. acetoin, diacetyl and 2-heptanone), all of which have been associated with desirable and more mature flavours in cheese. As such the production of a larger number of volatile compounds is a distinguishing feature of plant-derived lactococci and might be a desirable trait for the production of dairy products with enhanced flavour and/or aroma.en_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.relation.ispartofseriesInternational Journal of Food Microbiology;172
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectLactococcus lactisen_US
dc.subjectSugar metabolismen_US
dc.subjectPlasmiden_US
dc.subjectPCRen_US
dc.subjectPFGEen_US
dc.subjectGas chromatography–mass spectrometry (GC–MS)en_US
dc.titleCharacterization of plant-derived lactococci on the basis of their volatile compounds profile when grown in milken_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.ijfoodmicro.2013.11.024
dc.contributor.sponsorIrish Dairy Levy Research Trusten_US
dc.source.volume172
dc.source.beginpage57-61
refterms.dateFOA2020-08-06T15:08:47Z


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