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dc.contributor.authorHassan, Musa*
dc.contributor.authorHanrahan, James P*
dc.contributor.authorGood, Barbara*
dc.contributor.authorMulcahy, Grace*
dc.contributor.authorSweeney, Torres*
dc.date.accessioned2012-11-27T14:51:47Z
dc.date.available2012-11-27T14:51:47Z
dc.date.issued2011-03-08
dc.identifier.citationMusa Hassan, James P Hanrahan, Barbara Good, Grace Mulcahy, Torres Sweeney. A differential interplay between the expression of Th1/Th2/Treg related cytokine genes in Teladorsagia circumcincta infected DRB1*1101 carrier lambs. Veterinary Research, 2011, 42:45. doi:10.1186/1297-9716-42-45en_GB
dc.identifier.issn1297-9716
dc.identifier.urihttp://hdl.handle.net/11019/227
dc.descriptionpeer-reviewed
dc.description.abstractSubstantial debate exists on whether the immune response between sheep resistant and susceptible to gastrointestinal nematodes can be differentiated into a Th1 and Th2 phenotype. The present study addresses the hypothesis that variation in resistance to Teladorsagia circumcincta between DRB1*1101 (associated with reduced faecal egg count and worm burden) carriers and non-carriers is due to a differential interplay in the expression of Th1/Th2 and regulatory T (Treg) related cytokine genes. Lambs from each genotype were either slaughtered at day 0 (un-infected control) or infected with 3 × 104 Teladorsagia circumcincta L3 and slaughtered at 3, 7, 21, and 35 days later. Lambs carrying the DRB1*1101 allele had a significantly lower worm burden (P < 0.05) compared to the non-carriers. Abomasal mucosal cytokine gene expression was evaluated by quantitative real-time PCR and comparison made for time and genotype effects. The response generated varied through the course of infection and was affected by genotype. DRB1*1101 carriers had an up-regulated expression of the Th1-related cytokine genes (IL-1β, TNFα, and IFN-γ) at day 3, but this was replaced by an up-regulated expression of Th2-related cytokine genes (IL-10 and IL-13) and Treg-related cytokine genes (IL-2RA-CD25, TGFα, TGFβ, Arg2, MIF and FOXP3) by day 7. Conversely, in the non-carriers these changes in gene expression were delayed until days 7 and 21 post infection (pi), respectively. It is concluded that resistance to Teladorsagia circumcincta in animals carrying the DRB1*1101 allele is influenced by an earlier interplay between Th1, Th2 and T regulatory immune response genes.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Central
dc.relation.ispartofseriesVeterinary Research
dc.subjectTeladorsagia circumcincta
dc.subjectGene expression
dc.titleA differential interplay between the expression of Th1/Th2/Treg related cytokine genes in Teladorsagia circumcincta infected DRB1*1101 carrier lambsen_GB
dc.typeArticleen_GB
dc.identifier.rmisAYAB-0204-5685
dc.identifier.doihttp://hdl.handle.net/11019/227
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
refterms.dateFOA2018-01-12T07:31:29Z


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