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dc.contributor.authorKeogh, Kate
dc.contributor.authorWaters, Sinead M.
dc.contributor.authorCormican, Paul
dc.contributor.authorKelly, Alan K.
dc.contributor.authorKenny, David A.
dc.date.accessioned2020-08-19T16:01:29Z
dc.date.available2020-08-19T16:01:29Z
dc.date.issued2018-03-19
dc.identifier.citationKeogh K, Waters SM, Cormican P, Kelly AK, Kenny DA (2018) Effect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epithelium. PLoS ONE 13(3): e0194445. https://doi.org/ 10.1371/journal.pone.0194445en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11019/2297
dc.descriptionpeer-revieweden_US
dc.description.abstractCompensatory growth (CG), an accelerated growth phenomenon which occurs following a period of dietary restriction is utilised worldwide in animal production systems as a management practise to lower feed costs. The objective of this study was to evaluate the contribution of jejunal epithelial to CG in cattle through transcriptional profiling following a period of dietary restriction as well as subsequent re-alimentation induced CG. Sixty Holstein Friesian bulls were separated into two groups; RES and ADLIB, with 30 animals in each. RES animals were offered a restricted diet for 125 days (Period 1) followed by ad libitum feeding for 55 days (Period 2). ADLIB animals had ad libitum access to feed across both periods 1 and 2. At the end of each period, 15 animals from each treatment group were slaughtered, jejunal epithelium collected and RNAseq analysis performed. Animals that were previously diet restricted underwent CG, gaining 1.8 times the rate of their non-restricted counterparts. Twenty-four genes were differentially expressed in RES compared to ADLIB animals at the end of Period 1, with only one gene, GSTA1, differentially expressed between the two groups at the end of Period 2. When analysed within treatment (RES, Period 2 v Period 1), 31 genes were differentially expressed between diet restricted and animals undergoing CG. Dietary restriction and subsequent re-alimentation were associated with altered expression of genes involved in digestion and metabolism as well as those involved in cellular division and growth. Compensatory growth was also associated with greater expression of genes involved in cellular protection and detoxification in jejunal epithelium. This study highlights some of the molecular mechanisms regulating the response to dietary restriction and subsequent re-alimentation induced CG in cattle; however the gene expression results suggest that most of the CG in jejunal epithelium had occurred by day 55 of re-alimentation.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)en_US
dc.relation.ispartofseriesPLoS ONE;13
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectdieten_US
dc.subjectgene expressionen_US
dc.subjectjejunumen_US
dc.subjectnutrientsen_US
dc.subjectgastrointestinal tracten_US
dc.subjectepitheliumen_US
dc.subjectRNA sequencingen_US
dc.subjectcattleen_US
dc.titleEffect of dietary restriction and subsequent re-alimentation on the transcriptional profile of bovine jejunal epitheliumen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0194445
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorGrantNumber09/ RFP/GEN2447en_US
dc.source.volume13
dc.source.issue3
dc.source.beginpagee0194445
refterms.dateFOA2020-08-19T16:01:29Z


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