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dc.contributor.authorRosberg-Cody, Eva*
dc.contributor.authorLiavonchanka, Alena*
dc.contributor.authorGobel, Cornelia*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorO'Sullivan, Orla*
dc.contributor.authorFitzgerald, Gerald F*
dc.contributor.authorFeussner, Ivo*
dc.contributor.authorStanton, Catherine*
dc.date.accessioned2012-11-27T15:40:22Z
dc.date.available2012-11-27T15:40:22Z
dc.date.issued17/02/2011
dc.identifier.citationRosberg-Cody, Eva et al. Myosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protection. BMC Biochemistry, 2011, 12(1):9. DOI:10.1186/1471-2091-12-9en_GB
dc.identifier.issn1471-2091
dc.identifier.urihttp://hdl.handle.net/11019/229
dc.descriptionpeer-reviewed
dc.description.abstractBackground The aim of this study was to determine the catalytic activity and physiological role of myosin-cross-reactive antigen (MCRA) from Bifidobacterium breve NCIMB 702258. MCRA from B. breve NCIMB 702258 was cloned, sequenced and expressed in heterologous hosts (Lactococcus and Corynebacterium) and the recombinant proteins assessed for enzymatic activity against fatty acid substrates. Results MCRA catalysed the conversion of palmitoleic, oleic and linoleic acids to the corresponding 10-hydroxy fatty acids, but shorter chain fatty acids were not used as substrates, while the presence of trans-double bonds and double bonds beyond the position C12 abolished hydratase activity. The hydroxy fatty acids produced were not metabolised further. We also found that heterologous Lactococcus and Corynebacterium expressing MCRA accumulated increasing amounts of 10-HOA and 10-HOE in the culture medium. Furthermore, the heterologous cultures exhibited less sensitivity to heat and solvent stresses compared to corresponding controls. Conclusions MCRA protein in B. breve can be classified as a FAD-containing double bond hydratase, within the carbon-oxygen lyase family, which may be catalysing the first step in conjugated linoleic acid (CLA) production, and this protein has an additional function in bacterial stress protection.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Central
dc.relation.ispartofseriesBMC Biochemistry
dc.subjectMyosin-cross-reactive antigen
dc.subjectConjugated linoleic acid
dc.subjectBacterial stress protection
dc.titleMyosin-cross-reactive antigen (MCRA) protein from Bifidobacterium breve is a FAD-dependent fatty acid hydratase which has a function in stress protectionen_GB
dc.typeArticleen_GB
dc.identifier.rmisMDBY-0106-5274
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2091-12-9
refterms.dateFOA2018-01-12T07:25:36Z


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