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dc.contributor.authorLynch, David
dc.contributor.authorO’Connor, Paula M.
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorField, Des
dc.contributor.authorBegley, Máire
dc.date.accessioned2020-08-20T15:27:57Z
dc.date.available2020-08-20T15:27:57Z
dc.date.issued2019-10-16
dc.identifier.citationLynch D, O’Connor PM, Cotter PD, Hill C, Field D, Begley M (2019) Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis. PLoS ONE 14(10): e0223541. https://doi.org/10.1371/journal.pone.0223541en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11019/2302
dc.descriptionpeer-revieweden_US
dc.description.abstractOne hundred human-derived coagulase negative staphylococci (CoNS) were screened for antimicrobial activity using agar-based deferred antagonism assays with a range of indicator bacteria. Based on the findings of the screen and subsequent well assays with cell free supernatants and whole cell extracts, one strain, designated CIT060, was selected for further investigation. It was identified as Staphylococcus capitis and herein we describe the purification and characterisation of the novel bacteriocin that the strain produces. This bacteriocin which we have named capidermicin was extracted from the cell-free supernatant of S. capitis CIT060 and purified to homogeneity using reversed-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric (MS) analysis revealed that the capidermicin peptide has a mass of 5,464 Da. Minimal inhibitory concentration (MIC) experiments showed that capidermicin was active in the micro-molar range against all the Gram-positive bacteria that were tested. Antimicrobial activity was retained over a range of pHs (2–11) and temperatures (10–121°C x 15 mins). The draft genome sequence of S. capitis CIT060 was determined and the genes predicted to be involved in the biosynthesis of capidermicin were identified. These genes included the predicted capidermicin precursor gene, and genes that are predicted to encode a membrane transporter, an immunity protein and a transcriptional regulator. Homology searches suggest that capidermicin is a novel member of the family of class II leaderless bacteriocins.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Science (PLoS)en_US
dc.relation.ispartofseriesPLoS ONE;14
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectgut bacteriaen_US
dc.subjectlactococcus lactisen_US
dc.subjectgene predictionen_US
dc.subjectstaphylococcus epidermisen_US
dc.subjectstaphylococcus aureusen_US
dc.subjectstaphylococcusen_US
dc.subjectbacterial genomicsen_US
dc.titleIdentification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitisen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0223541
dc.contributor.sponsorCork Institute of Technology RISAM PhD scholarshipen_US
dc.source.volume14
dc.source.issue10
dc.source.beginpagee0223541
refterms.dateFOA2020-08-20T15:27:57Z


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