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dc.contributor.authorLane, Johnathan A.
dc.contributor.authorCalonne, Julie
dc.contributor.authorSlattery, Helen
dc.contributor.authorHickey, Rita M.
dc.date.accessioned2020-08-28T11:37:35Z
dc.date.available2020-08-28T11:37:35Z
dc.date.issued2019-10-01
dc.identifier.citationLane, J.A.; Calonne, J.; Slattery, H.; Hickey, R.M. Oligosaccharides Isolated from MGO™ Manuka Honey Inhibit the Adhesion of Pseudomonas aeruginosa, Escherichia Coli O157:H7 and Staphylococcus Aureus to Human HT-29 cells. Foods 2019, 8, 446. https://doi.org/10.3390/foods8100446en_US
dc.identifier.issn2304-8158
dc.identifier.urihttp://hdl.handle.net/11019/2318
dc.descriptionpeer-revieweden_US
dc.description.abstractHistorically, honey is known for its anti-bacterial and anti-fungal activities and its use for treatment of wound infections. Although this practice has been in place for millennia, little information exists regarding which manuka honey components contribute to the protective nature of this product. Given that sugar accounts for over 80% of honey and up to 25% of this sugar is composed of oligosaccharides, we have investigated the anti-infective activity of manuka honey oligosaccharides against a range of pathogens. Initially, oligosaccharides were extracted from a commercially-available New Zealand manuka honey—MGO™ Manuka Honey (Manuka Health New Zealand Ltd.)—and characterized by High pH anion exchange chromatography coupled with pulsed amperiometric detection. The adhesion of specific pathogens to the human colonic adenocarcinoma cell line, HT-29, was then assessed in the presence and absence of these oligosaccharides. Manuka honey oligosaccharides significantly reduced the adhesion of Escherichia coli O157:H7 (by 40%), Staphylococcus aureus (by 30%), and Pseudomonas aeruginosa (by 52%) to HT-29 cells. This activity was then proven to be concentration dependent and independent of bacterial killing. This study identifies MGO™ Manuka Honey as a source of anti-infective oligosaccharides for applications in functional foods aimed at lowering the incidence of infectious diseases.en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesFoods;8
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectManuka honeyen_US
dc.subjectoligosaccharidesen_US
dc.subjectanti-adhesionen_US
dc.subjectEscherichia colien_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectStaphylococcus aureusen_US
dc.titleOligosaccharides Isolated from MGO™ Manuka Honey Inhibit the Adhesion of Pseudomonas aeruginosa, Escherichia Coli O157:H7 and Staphylococcus Aureus to Human HT-29 cellsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/foods8100446
dc.source.volume8
dc.source.issue10
dc.source.beginpage446
refterms.dateFOA2020-08-28T11:37:36Z


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