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dc.contributor.authorQuinn, Erinn M.
dc.contributor.authorKilcoyne, Michelle
dc.contributor.authorWalsh, Dan
dc.contributor.authorJoshi, Lokesh
dc.contributor.authorHickey, Rita M.
dc.date.accessioned2020-11-03T17:06:36Z
dc.date.available2020-11-03T17:06:36Z
dc.date.issued2020-06-29
dc.identifier.citationQuinn, E.M.; Kilcoyne, M.; Walsh, D.; Joshi, L.; Hickey, R.M. A Whey Fraction Rich in Immunoglobulin G Combined with Bifidobacterium longum subsp. infantis ATCC 15697 Exhibits Synergistic Effects against Campylobacter jejuni. Int. J. Mol. Sci. 2020, 21, 4632. https://doi.org/10.3390/ijms21134632en_US
dc.identifier.urihttp://hdl.handle.net/11019/2352
dc.descriptionpeer-revieweden_US
dc.description.abstractEvidence that whey proteins and peptides have health benefits beyond basic infant nutrition has increased dramatically in recent years. Previously, we demonstrated that a whey-derived immunoglobulin G-enriched powder (IGEP) enhanced adhesion of Bifidobacterium longum subsp. infantis ATCC 15697 (B. infantis) to HT-29 cells. In this study, we investigated the synergistic effect of IGEP-treated B. infantis on preventing the attachment of highly invasive Campylobacter jejuni 81–176 (C. jejuni) to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 48% compared to the control (non-IGEP-treated B. infantis). We also confirmed that treatment of IGEP with sodium metaperiodate, which disables the biological recognition of the conjugated oligosaccharides, reduced adhesion of B. infantis to the intestinal cells. Thus, glycosylation of the IGEP components may be important in enhancing B. infantis adhesion. Interestingly, an increased adhesion phenotype was not observed when B. infantis was treated with bovine serum-derived IgG, suggesting that bioactivity was unique to milk-derived immunoglobulin-rich powders. Notably, IGEP did not induce growth of B. infantis within a 24 hours incubation period, as demonstrated by growth curves and metabolite analysis. The current study provides insight into the functionality of bovine whey components and highlights their potential in positively impacting the development of a healthy microbiota.en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesInternational Journal of Molecular Science;4632
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBifidobacteriumen_US
dc.subjectCampylobacteriaen_US
dc.subjectadhesionen_US
dc.subjectimmunoglobulinsen_US
dc.subjectsynbioticsen_US
dc.subjectHT-29 cellsen_US
dc.titleA Whey Fraction Rich in Immunoglobulin G Combined with Bifidobacterium longum subsp. infantis ATCC 15697 Exhibits Synergistic Effects against Campylobacter jejunien_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/ijms21134632
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.source.volume21
dc.source.issue13
dc.source.beginpage4632
refterms.dateFOA2020-11-03T17:06:37Z
dc.source.journaltitleInternational Journal of Molecular Sciences


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