Show simple item record

dc.contributor.authorWang, Shuo
dc.contributor.authorYang, Bo
dc.contributor.authorRoss, R. Paul
dc.contributor.authorSTANTON, CATHERINE
dc.contributor.authorZhao, Jianxin
dc.contributor.authorZhang, Hao
dc.contributor.authorChen, Wei
dc.date.accessioned2021-01-07T16:14:33Z
dc.date.available2021-01-07T16:14:33Z
dc.date.issued2020-01-08
dc.identifier.citationWang, S.; Yang, B.; Ross, R.P.; Stanton, C.; Zhao, J.; Zhang, H.; Chen, W. Comparative Genomics Analysis of Lactobacillus ruminis from Different Niches. Genes 2020, 11, 70. https://doi.org/10.3390/genes11010070en_US
dc.identifier.urihttp://hdl.handle.net/11019/2358
dc.descriptionpeer-revieweden_US
dc.description.abstractLactobacillus ruminis is a commensal motile lactic acid bacterium living in the intestinal tract of humans and animals. Although a few genomes of L. ruminis were published, most of them were animal derived. To explore the genetic diversity and potential niche-specific adaptation changes of L. ruminis, in the current work, draft genomes of 81 L. ruminis strains isolated from human, bovine, piglet, and other animals were sequenced, and comparative genomic analysis was performed. The genome size and GC content of L. ruminis on average were 2.16 Mb and 43.65%, respectively. Both the origin and the sampling distance of these strains had a great influence on the phylogenetic relationship. For carbohydrate utilization, the human-derived L. ruminis strains had a higher consistency in the utilization of carbon source compared to the animal-derived strains. L. ruminis mainly increased the competitiveness of niches by producing class II bacteriocins. The type of clustered regularly interspaced short palindromic repeats /CRISPR-associated (CRISPR/Cas) system presented in L. ruminis was mainly subtype IIA. The diversity of CRISPR/Cas locus depended on the high denaturation of spacer number and sequence, although cas1 protein was relatively conservative. The genetic differences in those newly sequenced L. ruminis strains highlighted the gene gains and losses attributed to niche adaptations.en_US
dc.description.sponsorshipInnovative Research Group Project of the National Natural Science Foundation of China
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesGenes;70
dc.rightsAttribution-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/*
dc.subjectLactobacillus ruminisen_US
dc.subjectphylogenetic relationshipen_US
dc.subjectbacteriocinsen_US
dc.subjectcarbohydrate utilizationen_US
dc.subjectCRISPR/Casen_US
dc.subjectprophageen_US
dc.titleComparative Genomics Analysis of Lactobacillus ruminis from Different Nichesen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/genes11010070
dc.contributor.sponsorNational Natural Science Foundation of Chinaen_US
dc.contributor.sponsorJiangsu Provinceen_US
dc.contributor.sponsorGrantNumber31771953en_US
dc.contributor.sponsorGrantNumber31820103010en_US
dc.contributor.sponsorGrantNumberJUFSTR20180102en_US
dc.source.volume11
dc.source.issue1
dc.source.beginpage70
refterms.dateFOA2021-01-07T16:14:40Z
dc.source.journaltitleGenes


Files in this item

Thumbnail
Name:
genes-11-00070.pdf
Size:
6.241Mb
Format:
PDF
Description:
main article

This item appears in the following Collection(s)

Show simple item record

Attribution-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-ShareAlike 4.0 International