The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins

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Author
Gaspard, Sophie cc
Brodkorb, Andre cc
Keyword
HPLC
Proteins
Denaturation
Aggregation
Reversed-phase chromatography
Size exclusion chromatography
Date
2019-07-25

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URI
http://hdl.handle.net/11019/2369
Citation
Gaspard S.J., Brodkorb A. (2019) The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins. In: McManus J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_8
Abstract
High-performance liquid chromatography (HPLC) is routinely used to identify and characterize proteins. HPLC can help to understand protein aggregation processes in dairy products, which are induced by common industrial processing steps such as heat treatment. In this chapter, three complementary chromatographic methods are described, which are based on the principles of size exclusion and reversed-phase chromatography. These methods are used to determine the degree of denaturation and aggregation of proteins, and estimate the molecular weight of these aggregates.
Funder
Dairy Levy Research Trust; Food Institutional Research Measure; Enterprise Ireland; Teagasc Walsh Fellowship Programme
Grant Number
MDDT6261; 08RDTMFRC650; CC20080001; 2012211
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