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dc.contributor.authorGaspard, Sophie
dc.contributor.authorBrodkorb, Andre
dc.date.accessioned2021-02-11T14:40:43Z
dc.date.available2021-02-11T14:40:43Z
dc.date.issued2019-07-25
dc.identifier.citationGaspard S.J., Brodkorb A. (2019) The Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteins. In: McManus J. (eds) Protein Self-Assembly. Methods in Molecular Biology, vol 2039. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9678-0_8en_US
dc.identifier.urihttp://hdl.handle.net/11019/2369
dc.descriptionpeer-revieweden_US
dc.description.abstractHigh-performance liquid chromatography (HPLC) is routinely used to identify and characterize proteins. HPLC can help to understand protein aggregation processes in dairy products, which are induced by common industrial processing steps such as heat treatment. In this chapter, three complementary chromatographic methods are described, which are based on the principles of size exclusion and reversed-phase chromatography. These methods are used to determine the degree of denaturation and aggregation of proteins, and estimate the molecular weight of these aggregates.en_US
dc.language.isoenen_US
dc.publisherSpringer New Yorken_US
dc.relation.ispartofseriesMethods in Molecular Biology;2039
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://www.springer.com/tdm
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectHPLCen_US
dc.subjectProteinsen_US
dc.subjectDenaturationen_US
dc.subjectAggregationen_US
dc.subjectReversed-phase chromatographyen_US
dc.subjectSize exclusion chromatographyen_US
dc.titleThe Use of High Performance Liquid Chromatography for the Characterization of the Unfolding and Aggregation of Dairy Proteinsen_US
dc.typeBook chapteren_US
dc.embargo.terms2021/07/31en_US
dc.identifier.doihttps://doi.org/10.1007/978-1-4939-9678-0_8
dc.contributor.sponsorDairy Levy Research Trusten_US
dc.contributor.sponsorFood Institutional Research Measureen_US
dc.contributor.sponsorEnterprise Irelanden_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumberMDDT6261en_US
dc.contributor.sponsorGrantNumber08RDTMFRC650en_US
dc.contributor.sponsorGrantNumberCC20080001en_US
dc.contributor.sponsorGrantNumber2012211en_US
dc.source.booktitleMethods in Molecular Biology
dc.source.booktitleProtein Self-Assembly
dc.source.beginpage103
dc.source.endpage115


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