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dc.contributor.authorO’Brien, Megan B.
dc.contributor.authorMcLoughlin, Rachel M.
dc.contributor.authorMeade, Kieran G.
dc.date.accessioned2021-02-17T17:24:04Z
dc.date.available2021-02-17T17:24:04Z
dc.date.issued2020-03
dc.identifier.citationM. B. O’Brien, R. M. McLoughlin, K. G. Meade, Application of the TruCulture® whole blood stimulation system for immune response profiling in cattle, Veterinary Immunology and Immunopathology, 2020, 221, 110025. doi: https://doi.org/10.1016/j.vetimm.2020.110025en_US
dc.identifier.urihttp://hdl.handle.net/11019/2386
dc.descriptionpeer-revieweden_US
dc.description.abstractCapturing the phenotypic variation in immune responses holds enormous promise for the development of targeted treatments for disease as well as tailored vaccination schedules. However, accurate detection of true biological variation can be obscured by the lack of standardised immune assays. The TruCulture® whole blood stimulation system has now been extensively used to detect basal and induced immune responses to a range of pathogen-associated molecular patterns (PAMPs) in human peripheral blood. This study demonstrates the optimisation of this commercially available assay for systemic immune phenotyping in cattle. The early immune response in Holstein-Friesian bull calves (n = 10) was assessed by haematology, flow cytometry and cytokine expression profiling after 24 h ex-vivo PAMP (LPS, poly (I:C) and zymosan) stimulation in TruCulture® tubes. A comparative analysis was also performed with a traditional whole blood stimulation assay and cell viability using both systems was also evaluated. Results: Supernatant collected from TruCulture® tubes showed a significant increase in IL-1β and IL-8 expression compared to null stimulated tubes in response to both LPS and zymosan. In contrast, a detectable immune response was not apparent at the standard concentration of poly (I:C). Conventional whole blood cultures yielded similar response profiles, although the magnitude of the response was higher to both LPS and zymosan, which may be attributed to prokaryotic strain-specificity or batch of the stimulant used. Despite being a closed system, HIF1A expression – used as a measure of hypoxia was not increased, suggesting the TruCulture® assay did not negatively affect cell viability. This represents the first reported use of this novel standardised assay in cattle, and indicates that the concentration of poly (I:C) immunogenic in humans is insufficient to induce cytokine responses in cattle. We conclude that the low blood volume and minimally invasive TruCulture® assay system offers a practical and informative technique to assess basal and induced systemic immune responses in cattle.en_US
dc.description.sponsorshipTeagasc Walsh Fellowship to MOB
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.relation.ispartofseriesVeterinary Immunology and Immunopathology;221
dc.rights© 2020 The Authors. Published by Elsevier B.V.
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectTruCulture®en_US
dc.subjectPAMPsen_US
dc.subjectBovine peripheral blooden_US
dc.subjectInnate immune responseen_US
dc.titleApplication of the TruCulture® whole blood stimulation system for immune response profiling in cattleen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.vetimm.2020.110025
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber0005GEen_US
dc.source.volume221
dc.source.beginpage110025
refterms.dateFOA2021-02-17T17:24:05Z
dc.source.journaltitleVeterinary Immunology and Immunopathology


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