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dc.contributor.authorO'Sullivan, Orla*
dc.contributor.authorO'Callaghan, John*
dc.contributor.authorSangrador-Vegas, Amaia*
dc.contributor.authorMcAuliffe, Olivia*
dc.contributor.authorSlattery, Lydia*
dc.contributor.authorKaleta, Pawel*
dc.contributor.authorCallanan, Michael J.*
dc.contributor.authorFitzgerald, Gerald F*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorBeresford, Tom*
dc.date.accessioned2012-11-29T12:08:30Z
dc.date.available2012-11-29T12:08:30Z
dc.date.issued05/03/2009
dc.identifier.citationOrla O'Sullivan, John O'Callaghan, Amaia Sangrador-Vegas, Olivia McAuliffe, Lydia Slattery, Pawel Kaleta, Michael Callanan, Gerald F Fitzgerald, R Paul Ross and Tom Beresford. Comparative genomics of lactic acid bacteria reveals a niche-specific gene set. BMC Microbiology. 2009 Mar 05;9(1):50. DOI:10.1186/1471-2180-9-50en_GB
dc.identifier.issn1471-2180
dc.identifier.urihttp://hdl.handle.net/11019/247
dc.descriptionpeer-reviewed
dc.description.abstractBackground: The recently sequenced genome of Lactobacillus helveticus DPC4571 1 revealed a dairy organism with significant homology (75% of genes are homologous) to a probiotic bacteria Lb. acidophilus NCFM 2. This led us to hypothesise that a group of genes could be determined which could define an organism's niche. Results: Taking 11 fully sequenced lactic acid bacteria (LAB) as our target, (3 dairy LAB, 5 gut LAB and 3 multi-niche LAB), we demonstrated that the presence or absence of certain genes involved in sugar metabolism, the proteolytic system, and restriction modification enzymes were pivotal in suggesting the niche of a strain. We identified 9 niche specific genes, of which 6 are dairy specific and 3 are gut specific. The dairy specific genes identified in Lactobacillus helveticus DPC4571 were lhv_1161 and lhv_1171, encoding components of the proteolytic system, lhv_1031 lhv_1152, lhv_1978 and lhv_0028 encoding restriction endonuclease genes, while bile salt hydrolase genes lba_0892 and lba_1078, and the sugar metabolism gene lba_1689 from Lb. acidophilus NCFM were identified as gut specific genes. Conclusion: Comparative analysis revealed that if an organism had homologs to the dairy specific geneset, it probably came from a dairy environment, whilst if it had homologs to gut specific genes, it was highly likely to be of intestinal origin. We propose that this "barcode" of 9 genes will be a useful initial guide to researchers in the LAB field to indicate an organism's ability to occupy a specific niche.en_GB
dc.language.isoenen_GB
dc.publisherBiomed Central
dc.relation.ispartofseriesBMC Microbiology
dc.subjectNiche specific genes
dc.subjectlactic acid bacteria
dc.titleComparative genomics of lactic acid bacteria reveals a niche-specific gene seten_GB
dc.typeArticleen_GB
dc.identifier.rmisMDAZ-0102-5434
dc.identifier.doihttp://dx.doi.org/10.1186/1471-2180-9-50
refterms.dateFOA2018-01-12T07:46:29Z


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