• Login
    View Item 
    •   T-Stór
    • Other
    • Teagasc publications in Biomed Central
    • View Item
    •   T-Stór
    • Other
    • Teagasc publications in Biomed Central
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of T-StórCommunitiesPublication DateAuthorsTitlesSubjectsFunderThis CollectionPublication DateAuthorsTitlesSubjectsFunderProfilesView

    My Account

    LoginRegister

    Information

    Deposit AgreementLicense

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.)

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    1471-2229-10-177.pdf
    Size:
    1.643Mb
    Format:
    PDF
    Download
    Thumbnail
    Name:
    1471-2229-10-177-S1.XLS
    Size:
    318Kb
    Format:
    Microsoft Excel
    Download
    Author
    Studer, Bruno
    Kolliker, Roland
    Muylle, Hilde
    Asp, Torben
    Frei, Ursula
    Roldan-Ruiz, Isabel
    Barre, Philippe
    Tomaszewski, Celine
    Meally, Helena
    Barth, Susanne cc
    Skot, Leif
    Armstead, Ian P
    Dolstra, Oene
    Lubberstedt, Thomas
    Show allShow less
    Keyword
    Lolium spp.
    Genetic linkage maps
    molecular breeding
    Ryegrass
    Date
    16/08/2010
    
    Metadata
    Show full item record
    Statistics
    Display Item Statistics
    URI
    http://hdl.handle.net/11019/249
    Citation
    Studer, B. et al. EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.). BMC Plant Biology, 2010, Aug 16;10(1):177. DOI:10.1186/1471-2229-10-177
    Abstract
    Background: Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations have been aligned with each other using publicly available DNA markers, the number of common markers among genetic maps is still low, limiting the ability to compare candidate gene and QTL locations across germplasm. Results: A set of 204 expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers has been assigned to map positions using eight different ryegrass mapping populations. Marker properties of a subset of 64 EST-SSRs were assessed in six to eight individuals of each mapping population and revealed 83% of the markers to be polymorphic in at least one population and an average number of alleles of 4.88. EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass. The integrated map was complemented with 97 SSRs from previously published linkage maps and finally contained 284 EST-derived and genomic SSR markers. The total map length was 742 centiMorgan (cM), ranging for individual chromosomes from 70 cM of linkage group (LG) 6 to 171 cM of LG 2. Conclusions: The consensus linkage map for ryegrass based on eight mapping populations and constructed using a large set of publicly available Lolium EST-SSRs mapped for the first time together with previously mapped SSR markers will allow for consolidating existing mapping and QTL information in ryegrass. Map and markers presented here will prove to be an asset in the development for both molecular breeding of ryegrass as well as comparative genetics and genomics within grass species.
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1186/1471-2229-10-177
    Scopus Count
    Collections
    Crop Science
    Teagasc publications in Biomed Central
    Teagasc publications in Biomed Central
    Teagasc publications in Biomed Central

    entitlement

     
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.