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dc.contributor.authorO’Callaghan, Tom
dc.contributor.authorVázquez-Fresno, Rosa
dc.contributor.authorSerra-Cayuela, Arnau
dc.contributor.authorDong, Edison
dc.contributor.authorMandal, Rupasri
dc.contributor.authorHennessy, Deirdre
dc.contributor.authorMcAuliffe, Stephen
dc.contributor.authorDillon, Pat
dc.contributor.authorWishart, David
dc.contributor.authorStanton, Catherine
dc.contributor.authorRoss, R.
dc.date.accessioned2021-08-10T11:51:50Z
dc.date.available2021-08-10T11:51:50Z
dc.date.issued2018-04-06
dc.identifier.citationO’Callaghan, T.F.; Vázquez-Fresno, R.; Serra-Cayuela, A.; Dong, E.; Mandal, R.; Hennessy, D.; McAuliffe, S.; Dillon, P.; Wishart, D.S.; Stanton, C.; Ross, R.P. Pasture Feeding Changes the Bovine Rumen and Milk Metabolome. Metabolites 2018, 8, 27. https://doi.org/10.3390/metabo8020027en_US
dc.identifier.issn2218-1989
dc.identifier.urihttp://hdl.handle.net/11019/2553
dc.descriptionpeer-revieweden_US
dc.description.abstractThe purpose of this study was to examine the effects of two pasture feeding systems—perennial ryegrass (GRS) and perennial ryegrass and white clover (CLV)—and an indoor total mixed ration (TMR) system on the (a) rumen microbiome; (b) rumen fluid and milk metabolome; and (c) to assess the potential to distinguish milk from different feeding systems by their respective metabolomes. Rumen fluid was collected from nine rumen cannulated cows under the different feeding systems in early, mid and late lactation, and raw milk samples were collected from ten non-cannulated cows in mid-lactation from each of the feeding systems. The microbiota present in rumen liquid and solid portions were analysed using 16S rRNA gene sequencing, while 1H-NMR untargeted metabolomic analysis was performed on rumen fluid and raw milk samples. The rumen microbiota composition was not found to be significantly altered by any feeding system in this study, likely as a result of a shortened adaptation period (two weeks’ exposure time). In contrast, feeding system had a significant effect on both the rumen and milk metabolome. Increased concentrations of volatile fatty acids including acetic acid, an important source of energy for the cow, were detected in the rumen of TMR and CLV-fed cows. Pasture feeding resulted in significantly higher concentrations of isoacids in the rumen. The ruminal fluids of both CLV and GRS-fed cows were found to have increased concentrations of p-cresol, a product of microbiome metabolism. CLV feeding resulted in increased rumen concentrations of formate, a substrate compound for methanogenesis. The TMR feeding resulted in significantly higher rumen choline content, which contributes to animal health and milk production, and succinate, a product of carbohydrate metabolism. Milk and rumen-fluids were shown to have varying levels of dimethyl sulfone in each feeding system, which was found to be an important compound for distinguishing between the diets. CLV feeding resulted in increased concentrations of milk urea. Milk from pasture-based feeding systems was shown to have significantly higher concentrations of hippuric acid, a potential biomarker of pasture-derived milk. This study has demonstrated that 1H-NMR metabolomics coupled with multivariate analysis is capable of distinguishing both rumen-fluid and milk derived from cows on different feeding systems, specifically between indoor TMR and pasture-based diets used in this study.en_US
dc.language.isoenen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofseriesMetabolites;8
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectcows dieten_US
dc.subjectrumenen_US
dc.subjectmilken_US
dc.subjectmetabolomeen_US
dc.subjectpastureen_US
dc.subjecttotal mixed rationen_US
dc.titlePasture Feeding Changes the Bovine Rumen and Milk Metabolomeen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/metabo8020027
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorDairy Research Irelanden_US
dc.contributor.sponsorTeagasc Walsh Scholarship Programmeen_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
dc.source.volume8
dc.source.issue2
dc.source.beginpage27
refterms.dateFOA2021-08-10T11:51:51Z


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