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dc.contributor.authorMurphy, Caoileann H
dc.contributor.authorFlanagan, Ellen M
dc.contributor.authorDe Vito, Giuseppe
dc.contributor.authorSusta, Davide
dc.contributor.authorMitchelson, Kathleen A J
dc.contributor.authorde Marco Castro, Elena
dc.contributor.authorSenden, Joan M G
dc.contributor.authorGoessens, Joy P B
dc.contributor.authorMikłosz, Agnieszka
dc.contributor.authorChabowski, Adrian
dc.contributor.authorSegurado, Ricardo
dc.contributor.authorCorish, Clare A
dc.contributor.authorMcCarthy, Sinead N.
dc.contributor.authorEgan, Brendan
dc.contributor.authorvan Loon, Luc J C
dc.contributor.authorRoche, Helen M
dc.identifier.citationCaoileann H Murphy, Ellen M Flanagan, Giuseppe De Vito, Davide Susta, Kathleen A J Mitchelson, Elena de Marco Castro, Joan M G Senden, Joy P B Goessens, Agnieszka Mikłosz, Adrian Chabowski, Ricardo Segurado, Clare A Corish, Sinead N McCarthy, Brendan Egan, Luc J C van Loon, Helen M Roche, Does supplementation with leucine-enriched protein alone and in combination with fish-oil-derived n–3 PUFA affect muscle mass, strength, physical performance, and muscle protein synthesis in well-nourished older adults? A randomized, double-blind, placebo-controlled trial, The American Journal of Clinical Nutrition, Volume 113, Issue 6, June 2021, Pages 1411–1427,
dc.description.abstractBackground Leucine-enriched protein (LEU-PRO) and long-chain (LC) n–3 (ω–3) PUFAs have each been proposed to improve muscle mass and function in older adults, whereas their combination may be more effective than either alone. Objective The impact of LEU-PRO supplementation alone and combined with LC n–3 PUFAs on appendicular lean mass, strength, physical performance and myofibrillar protein synthesis (MyoPS) was investigated in older adults at risk of sarcopenia. Methods This 24-wk, 3-arm parallel, randomized, double-blind, placebo-controlled trial was conducted in 107 men and women aged ≥65 y with low muscle mass and/or strength. Twice daily, participants consumed a supplement containing either LEU-PRO (3 g leucine, 10 g protein; n = 38), LEU-PRO plus LC n–3 PUFAs (0.8 g EPA, 1.1 g DHA; LEU-PRO+n–3; n = 38), or an isoenergetic control (CON; n = 31). Appendicular lean mass, handgrip strength, leg strength, physical performance, and circulating metabolic and renal function markers were measured pre-, mid-, and postintervention. Integrated rates of MyoPS were assessed in a subcohort (n = 28). Results Neither LEU-PRO nor LEU-PRO+n–3 supplementation affected appendicular lean mass, handgrip strength, knee extension strength, physical performance or MyoPS. However, isometric knee flexion peak torque (treatment effect: −7.1 Nm; 95% CI: −12.5, −1.8 Nm; P < 0.01) was lower postsupplementation in LEU-PRO+n–3 compared with CON. Serum triacylglycerol and total adiponectin concentrations were lower, and HOMA-IR was higher, in LEU-PRO+n–3 compared with CON postsupplementation (all P < 0.05). Estimated glomerular filtration rate was higher and cystatin c was lower in LEU-PRO and LEU-PRO+n–3 postsupplementation compared with CON (all P < 0.05). Conclusions Contrary to our hypothesis, we did not observe a beneficial effect of LEU-PRO supplementation alone or combined with LC n–3 PUFA supplementation on appendicular lean mass, strength, physical performance or MyoPS in older adults at risk of sarcopenia. This trial was registered at as NCT03429491.en_US
dc.description.sponsorshipHorizon 2020 Framework Programme
dc.description.sponsorshipThis work was supported by the Department of Agriculture, Food and the Marine Food Institutional Research Measure grant entitled NUTRIMAL “Novel Nutritional Solutions for the Prevention of Malnutrition” (grant 14F822), the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie Grant Agreement No. 666010, and a Research Fellowship awarded to CHM by the European Society of Clinical Nutrition and Metabolism (ESPEN). HMR was supported by funding from the Joint Programming Initiative Healthy Diet for a Healthy Life (JPI HDHL) EU Food Biomarkers Alliance “FOODBAll” with Science Foundation Ireland (14/JPHDHL/B3076).
dc.publisherOxford University Press (OUP)en_US
dc.relation.ispartofseriesThe American Journal of Clinical Nutrition;
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectLC n–3 PUFAen_US
dc.subjectmuscle massen_US
dc.subjectpolyunsaturated fatty acidsen_US
dc.subjectolder adulten_US
dc.titleDoes supplementation with leucine-enriched protein alone and in combination with fish-oil-derived n–3 PUFA affect muscle mass, strength, physical performance, and muscle protein synthesis in well-nourished older adults? A randomized, double-blind, placebo-controlled trialen_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorEuropean Unionen_US
dc.contributor.sponsorEuropean Society of Clinical Nutrition and Metabolismen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.source.journaltitleThe American Journal of Clinical Nutrition

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