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dc.contributor.authorLinnane, Barry
dc.contributor.authorWalsh, Aaron M.
dc.contributor.authorWalsh, Calum J.
dc.contributor.authorCrispie, Fiona
dc.contributor.authorO’Sullivan, Orla
dc.contributor.authorCotter, Paul D.
dc.contributor.authorMcDermott, Michael
dc.contributor.authorRenwick, Julie
dc.contributor.authorMcNally, Paul
dc.date.accessioned2021-11-16T15:53:30Z
dc.date.available2021-11-16T15:53:30Z
dc.date.issued2021-02-26
dc.identifier.citationLinnane B, Walsh AM, Walsh CJ, et al. The Lung Microbiome in Young Children with Cystic Fibrosis: A Prospective Cohort Study. Microorganisms 2021;9(3):492. doi: https://doi.org/10.3390/microorganisms9030492en_US
dc.identifier.urihttp://hdl.handle.net/11019/2622
dc.descriptionpeer revieweden_US
dc.description.abstractThe cystic fibrosis (CF) lung harbours a diverse microbiome and reduced diversity in the CF lung has been associated with advancing age, increased inflammation and poorer lung function. Data suggest that the window for intervention is early in CF, yet there is a paucity of studies on the lung microbiome in children with CF. The objective of this study was to thoroughly characterise the lower airway microbiome in pre-school children with CF. Bronchoalveolar lavage (BAL) samples were collected annually from children attending the three clinical centres. Clinical and demographic data were collated on all subjects alongside BAL inflammatory markers. 16S rRNA gene sequencing was performed on the Illumina MiSeq platform. Bioinformatics and data analysis were performed using Qiime and R project software. Data on 292 sequenced BALs from 101 children with CF and 51 without CF show the CF lung microbiome, while broadly similar to that in non-CF children, is distinct. Alpha diversity between the two cohorts was indistinguishable at this early age. The CF diagnosis explained only 1.1% of the variation between the cohort microbiomes. However, several key genera were significantly differentially abundant between the groups. While the non-CF lung microbiome diversity increased with age, diversity reduced in CF with age. Pseudomonas and Staphylococcus were more abundant with age, while genera such as Streptococcus, Porphyromonas and Veillonella were less abundant with age. There was a negative correlation between alpha diversity and interleukin-8 and neutrophil elastase in the CF population. Neither current flucloxacillin or azithromycin prophylaxis, nor previous oral or IV antibiotic exposure, was correlated with microbiome diversity. Consecutive annual BAL samples over 5 years from a subgroup of children demonstrated diverse patterns of development in the first years of life.en_US
dc.description.sponsorshipNational Children's Research Centre
dc.language.isoenen_US
dc.publisherMultidisciplinary Digital Publishing Instituteen_US
dc.relation.ispartofseriesMicroorganisms;Vol. 9
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectInfectionen_US
dc.subjectinflammationen_US
dc.subjectmicrobiotaen_US
dc.subjectmicrobiomeen_US
dc.subjectlungen_US
dc.subjectcystic fibrosisen_US
dc.subjectchildrenen_US
dc.subjectpaediatricsen_US
dc.subjectbronchoscopyen_US
dc.subjectbronchoalveolar lavageen_US
dc.titleThe Lung Microbiome in Young Children with Cystic Fibrosis: A Prospective Cohort Studyen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/microorganisms9030492
dc.identifier.piimicroorganisms9030492
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorNational Children's Hospital, Crumlinen_US
dc.contributor.sponsorNational Children's Hospital, Tallaghten_US
dc.contributor.sponsorGrantNumberSFI/12/RC/2273en_US
dc.contributor.sponsorGrantNumber11/PI/1137en_US
dc.contributor.sponsorGrantNumber13/SIRG/2160en_US
dc.source.volume9
dc.source.issue3
dc.source.beginpage492
refterms.dateFOA2021-11-16T15:53:31Z
dc.source.journaltitleMicroorganisms
dc.identifier.eissn2076-2607


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Attribution-NonCommercial-ShareAlike 4.0 International
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