O’Connor, Paula M.
Walsh, Calum J.
Hegarty, James W.
Guinane, Caitriona M.
Mayer, Melinda J.
Cotter, Paul D.
Streptococcus agalactiae DPC7040
minimum inhibitory concentration (MIC)
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CitationGarcia-Gutierrez E, O’Connor PM, Saalbach G, et al. First evidence of production of the lantibiotic nisin P. Scientific Reports 2020;10:3738. doi:10.1038/s41598-020-60623-0
AbstractNisin P is a natural nisin variant, the genetic determinants for which were previously identified in the genomes of two Streptococcus species, albeit with no confirmed evidence of production. Here we describe Streptococcus agalactiae DPC7040, a human faecal isolate, which exhibits antimicrobial activity against a panel of gut and food isolates by virtue of producing nisin P. Nisin P was purified, and its predicted structure was confirmed by nanoLC-MS/MS, with both the fully modified peptide and a variant without rings B and E being identified. Additionally, we compared its spectrum of inhibition and minimum inhibitory concentration (MIC) with that of nisin A and its antimicrobial effect in a faecal fermentation in comparison with nisin A and H. We found that its antimicrobial activity was less potent than nisin A and H, and we propose a link between this reduced activity and the peptide structure.
FunderTeagasc Walsh Scholarship Programme; BBSRC Institute Strategic Programme; Science Foundation Ireland
Grant Number2015066; BB/R012490/1; BBS/E/F/000PR10356; SFI/11/PI/1137; SFI/12/RC/2273
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