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dc.contributor.authorMunita, Maria Pia
dc.contributor.authorRea, Rosemary
dc.contributor.authorMartinez-Ibeas, Ana Maria
dc.contributor.authorByrne, Noel
dc.contributor.authorKennedy, Aideen
dc.contributor.authorSekiya, Mary
dc.contributor.authorMulcahy, Grace
dc.contributor.authorSayers, Riona
dc.date.accessioned2021-12-09T15:11:14Z
dc.date.available2021-12-09T15:11:14Z
dc.date.issued2019-11-21
dc.identifier.citationMunita, M.P., Rea, R., Martinez-Ibeas, A.M. et al. Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle. BMC Vet Res 15, 414 (2019). https://doi.org/10.1186/s12917-019-2160-xen_US
dc.identifier.issn1746-6148
dc.identifier.urihttp://hdl.handle.net/11019/2696
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground: Fasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F. hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate. Results: In the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees. Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied. Conclusions: Se and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds. In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofseriesBMC Vet;414
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectFasciola hepaticaen_US
dc.subjectELISAen_US
dc.subjectComparisonen_US
dc.subjectSensitivityen_US
dc.subjectSpecificityen_US
dc.subjectTreatmenten_US
dc.subjectFlukicidesen_US
dc.subjectSeasonalityen_US
dc.titleComparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattleen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1186/s12917-019-2160-x
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorDairy Research Irelanden_US
dc.contributor.sponsorGrantNumber13/ S/405en_US
dc.source.volume15
dc.source.issue1
refterms.dateFOA2021-12-09T15:11:14Z


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