Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria
AuthorMorrin, Sinead T.
Irwin, Jane A.
Hickey, Rita M.
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CitationMorrin, S.T., McCarthy, G., Kennedy, D. et al. Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria. AMB Expr 10, 114 (2020). https://doi.org/10.1186/s13568-020-01048-w
AbstractA bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s responsible for driving the changes in the intestinal cells. The adherence of various bifdobacteria to HT-29 cells was increased when the intestinal cells were pre-incubated with immunoglobulin G (IgG). Modulation of the intestinal cells by IgG was concentration dependent with 16 mg/ mL IgG resulting in a 43-fold increase in the adhesion of Bifdobacterium longum NCIMB 8809 to HT-29 cells. Periodate treatment of colostral IgG prior to performing the colonization studies resulted in a reduction in the adhesion of the strain to the intestinal cells demonstrating that the glycans of IgG may be important in modulating the intestinal cells for enhanced commensal adhesion. IgG isolated from mature milk also resulted in signifcant increases in adhesion of the Bifdobacterium strains tested albeit at reduced levels (3.9-fold). The impact of IgG on the HT-29 cells was also visualised via scanning electron microscopy. This study builds a strong case for the inclusion of IgG ingredients sourced from cow’s milk in functional foods aimed at increasing numbers of health promoting bacteria in the human gut.
FunderTeagasc Walsh Fellowship Programme
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