Show simple item record

dc.contributor.authorMorrin, Sinead T.
dc.contributor.authorMcCarthy, Geoffrey
dc.contributor.authorKennedy, Deirdre
dc.contributor.authorMarotta, Mariarosaria
dc.contributor.authorIrwin, Jane A.
dc.contributor.authorHickey, Rita M.
dc.date.accessioned2021-12-14T12:41:15Z
dc.date.available2021-12-14T12:41:15Z
dc.date.issued2020-06-18
dc.identifier.citationMorrin, S.T., McCarthy, G., Kennedy, D. et al. Immunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteria. AMB Expr 10, 114 (2020). https://doi.org/10.1186/s13568-020-01048-wen_US
dc.identifier.issn2191-0855
dc.identifier.urihttp://hdl.handle.net/11019/2698
dc.descriptionpeer-revieweden_US
dc.description.abstractA bovine colostrum fraction (BCF) was recently shown to enhance the adherence of several commensal organisms to intestinal epithelial cells through modulating the epithelial cell surface. In this study, the main components of the BCF were examined to investigate the active component/s responsible for driving the changes in the intestinal cells. The adherence of various bifdobacteria to HT-29 cells was increased when the intestinal cells were pre-incubated with immunoglobulin G (IgG). Modulation of the intestinal cells by IgG was concentration dependent with 16 mg/ mL IgG resulting in a 43-fold increase in the adhesion of Bifdobacterium longum NCIMB 8809 to HT-29 cells. Periodate treatment of colostral IgG prior to performing the colonization studies resulted in a reduction in the adhesion of the strain to the intestinal cells demonstrating that the glycans of IgG may be important in modulating the intestinal cells for enhanced commensal adhesion. IgG isolated from mature milk also resulted in signifcant increases in adhesion of the Bifdobacterium strains tested albeit at reduced levels (3.9-fold). The impact of IgG on the HT-29 cells was also visualised via scanning electron microscopy. This study builds a strong case for the inclusion of IgG ingredients sourced from cow’s milk in functional foods aimed at increasing numbers of health promoting bacteria in the human gut.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofseriesAMB Express;114
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectImmunoglobulin Gen_US
dc.subjectMilken_US
dc.subjectBifdobacteriumen_US
dc.subjectGlycosylationen_US
dc.subjectAdhesionen_US
dc.titleImmunoglobulin G from bovine milk primes intestinal epithelial cells for increased colonization of bifidobacteriaen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1186/s13568-020-01048-w
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber2014058en_US
dc.source.volume10
dc.source.issue1
refterms.dateFOA2021-12-14T12:41:15Z


Files in this item

Thumbnail
Name:
s13568-020-01048-w.pdf
Size:
1.593Mb
Format:
PDF
Description:
main article

This item appears in the following Collection(s)

Show simple item record

Attribution-ShareAlike 3.0 United States
Except where otherwise noted, this item's license is described as Attribution-ShareAlike 3.0 United States