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dc.contributor.authorRex, David
dc.contributor.authorClough, Timothy J.
dc.contributor.authorRichards, Karl G.
dc.contributor.authorCondron, Leo M.
dc.contributor.authorde Klein, Cecile A. M.
dc.contributor.authorMorales, Sergio E.
dc.contributor.authorLanigan, Gary J.
dc.date.accessioned2021-12-16T14:35:02Z
dc.date.available2021-12-16T14:35:02Z
dc.date.issued2019-09-16
dc.identifier.citationRex, D., Clough, T.J., Richards, K.G. et al. Impact of nitrogen compounds on fungal and bacterial contributions to codenitrification in a pasture soil. Sci Rep 9, 13371 (2019). https://doi.org/10.1038/s41598-019-49989-yen_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11019/2702
dc.descriptionpeer-revieweden_US
dc.description.abstractRuminant urine patches on grazed grassland are a signifcant source of agricultural nitrous oxide (N2O) emissions. Of the many biotic and abiotic N2O production mechanisms initiated following urine-urea deposition, codenitrifcation resulting in the formation of hybrid N2O, is one of the least understood. Codenitrifcation forms hybrid N2O via biotic N-nitrosation, co-metabolising organic and inorganic N compounds (N substrates) to produce N2O. The objective of this study was to assess the relative signifcance of diferent N substrates on codenitrifcation and to determine the contributions of fungi and bacteria to codenitrifcation. 15N-labelled ammonium, hydroxylamine (NH2OH) and two amino acids (phenylalanine or glycine) were applied, separately, to sieved soil mesocosms eight days after a simulated urine event, in the absence or presence of bacterial and fungal inhibitors. Soil chemical variables and N2O fuxes were monitored and the codenitrifed N2O fuxes determined. Fungal inhibition decreased N2O fuxes by ca. 40% for both amino acid treatments, while bacterial inhibition only decreased the N2O fux of the glycine treatment, by 14%. Hydroxylamine (NH2OH) generated the highest N2O fuxes which declined with either fungal or bacterial inhibition alone, while combined inhibition resulted in a 60% decrease in the N2O fux. All the N substrates examined participated to some extent in codenitrifcation. Trends for codenitrifcation under the NH2OH substrate treatment followed those of total N2O fuxes (85.7% of total N2O fux). Codenitrifcation fuxes under non-NH2OH substrate treatments (0.7–1.2% of total N2O fux) were two orders of magnitude lower, and signifcant decreases in these treatments only occurred with fungal inhibition in the amino acid substrate treatments. These results demonstrate that in situ studies are required to better understand the dynamics of codenitrifcation substrates in grazed pasture soils and the associated role that fungi have with respect to codenitrifcation.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofseriesScientific Reports;13371
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectClimate-change mitigationen_US
dc.subjectElement cyclesen_US
dc.subjectGrassland ecologyen_US
dc.subjectMicrobial ecologyen_US
dc.subjectStable isotope analysisen_US
dc.titleImpact of nitrogen compounds on fungal and bacterial contributions to codenitrification in a pasture soilen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1038/s41598-019-49989-y
dc.contributor.sponsorNew Zealand Governmenten_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programmeen_US
dc.contributor.sponsorGrantNumber16084en_US
dc.source.volume9
dc.source.issue1
refterms.dateFOA2021-12-16T14:35:03Z


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