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dc.contributor.authorJohnston, Dayle
dc.contributor.authorEarley, Bernadette
dc.contributor.authorMcCabe, Matthew S.
dc.contributor.authorLemon, Ken
dc.contributor.authorDuffy, Catherine
dc.contributor.authorMcMenamy, Michael
dc.contributor.authorCosby, S. Louise
dc.contributor.authorKim, JaeWoo
dc.contributor.authorBlackshields, Gordon
dc.contributor.authorTaylor, Jeremy F.
dc.contributor.authorWaters, Sinead M.
dc.date.accessioned2021-12-16T14:59:41Z
dc.date.available2021-12-16T14:59:41Z
dc.date.issued2019-10-14
dc.identifier.citationJohnston, D., Earley, B., McCabe, M.S. et al. Experimental challenge with bovine respiratory syncytial virus in dairy calves: bronchial lymph node transcriptome response. Sci Rep 9, 14736 (2019). https://doi.org/10.1038/s41598-019-51094-zen_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11019/2706
dc.descriptionpeer-revieweden_US
dc.description.abstractBovine Respiratory Disease (BRD) is the leading cause of mortality in calves. The objective of this study was to examine the response of the host’s bronchial lymph node transcriptome to Bovine Respiratory Syncytial Virus (BRSV) in a controlled viral challenge. Holstein-Friesian calves were either inoculated with virus (103.5TCID50/ml×15ml) (n=12) or mock challenged with phosphate bufered saline (n=6). Clinical signs were scored daily and blood was collected for haematology counts, until euthanasia at day 7 post-challenge. RNA was extracted and sequenced (75bp paired-end) from bronchial lymph nodes. Sequence reads were aligned to the UMD3.1 bovine reference genome and diferential gene expression analysis was performed using EdgeR. There was a clear separation between BRSV challenged and control calves based on gene expression changes, despite an observed mild clinical manifestation of the disease. Therefore, measuring host gene expression levels may be benefcial for the diagnosis of subclinical BRD. There were 934 diferentially expressed genes (DEG) (p<0.05, FDR <0.1, fold change >2) between the BRSV challenged and control calves. Over-represented gene ontology terms, pathways and molecular functions, among the DEG, were associated with immune responses. The top enriched pathways included interferon signaling, granzyme B signaling and pathogen pattern recognition receptors, which are responsible for the cytotoxic responses necessary to eliminate the virus.en_US
dc.language.isoenen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofseriesScientific Reports;14736
dc.rightsAttribution-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/3.0/us/*
dc.subjectTranscriptomicsen_US
dc.subjectViral infectionen_US
dc.titleExperimental challenge with bovine respiratory syncytial virus in dairy calves: bronchial lymph node transcriptome responseen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1038/s41598-019-51094-z
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorDepartment of Agriculture, Environment and Rural Affairs, Northern Irelanden_US
dc.contributor.sponsorUnited States Department for Agriculture National Institute for Food and Agricultureen_US
dc.contributor.sponsorGrantNumber16/RD/US-ROI/11en_US
dc.contributor.sponsorGrantNumber2017-67015-26760en_US
dc.source.volume9
dc.source.issue1
refterms.dateFOA2021-12-16T14:59:41Z


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