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dc.contributor.authorAbril-Parreño, Laura
dc.contributor.authorMeade, Kieran G.
dc.contributor.authorKrogenæs, Anette K.
dc.contributor.authorDruart, Xavier
dc.contributor.authorFair, Sean
dc.contributor.authorCormican, Paul
dc.date.accessioned2021-12-21T11:37:33Z
dc.date.available2021-12-21T11:37:33Z
dc.date.issued2021-10-20
dc.identifier.citationAbril-Parreño, L., Meade, K.G., Krogenæs, A.K. et al. Conserved and breed-specific differences in the cervical transcriptome of sheep with divergent fertility at the follicular phase of a natural oestrus cycle. BMC Genomics 22, 752 (2021). https://doi.org/10.1186/s12864-021-08060-9en_US
dc.identifier.urihttp://hdl.handle.net/11019/2725
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground The outcome of cervical artificial insemination (AI) with frozen-thawed semen in sheep is limited by the inability of sperm to traverse the cervix of some ewe breeds. Previous research has demonstrated that cervical sperm transport is dependent on ewe breed, as sperm can traverse the cervix in greater numbers in some higher fertility ewe breeds. However, the molecular mechanisms underlying ewe breed differences in sperm transport through the cervix remain unknown. In this study, we aimed to characterise the cervical transcriptome of four European ewe breeds with known differences in pregnancy rates following cervical AI using frozen-thawed semen at the follicular phase of a natural oestrous cycle. Cervical post mortem tissue samples were collected from two Irish ewe breeds (Belclare and Suffolk; medium and low fertility, respectively) and from two Norwegian ewe breeds (Norwegian White Sheep (NWS) and Fur; high fertility compared to both Irish breeds) at the follicular phase of a natural oestrous cycle (n = 8 to 10 ewes per breed). Results High-quality RNA extracted from biopsies of the mid-region of the cervix was analysed by RNA-sequencing and Gene Ontology (GO). After stringent filtering (P <  0.05 and FC > 1.5), a total of 11, 1539 and 748 differentially expressed genes (DEGs) were identified in Belclare, Fur and NWS compared to the low fertility Suffolk breed, respectively. Gene ontology analysis identified significantly enriched biological processes involved in muscle contraction, extracellular matrix (ECM) development and the immune response. Gene co-expression analysis revealed similar patterns in muscle contraction and ECM development modules in both Norwegian ewe breeds, which differed to the Irish ewe breeds. Conclusions These breed-specific biological processes may account for impaired cervical sperm transport through the cervix in sheep during the follicular phase of the reproductive cycle. This novel and comprehensive dataset provides a rich foundation for future targeted initiatives to improve cervical AI in sheep.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesBMC Genomics;
dc.subjectovineen_US
dc.subjectCervixen_US
dc.subjectRNA-sequencingen_US
dc.subjectFertilityen_US
dc.titleConserved and breed-specific differences in the cervical transcriptome of sheep with divergent fertility at the follicular phase of a natural oestrus cycleen_US
dc.typeArticleen_US
dc.date.updated2021-10-24T03:11:54Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.identifier.doihttps://doi.org/10.1186/s12864-021-08060-9
dc.contributor.sponsorEuropean Unionen_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorTeagascen_US
dc.contributor.sponsorResearch Council of Norwayen_US
dc.contributor.sponsorGrantNumber16/RD/SusAn/ERA-NETen_US
dc.contributor.sponsorGrantNumberNFR 272338 / E50en_US
refterms.dateFOA2021-12-21T11:37:33Z


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