• Login
    View Item 
    •   T-Stór
    • Other
    • Teagasc publications in Biomed Central
    • View Item
    •   T-Stór
    • Other
    • Teagasc publications in Biomed Central
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of T-StórCommunitiesPublication DateAuthorsTitlesSubjectsFunderThis CollectionPublication DateAuthorsTitlesSubjectsFunderProfilesView

    My Account

    LoginRegister

    Information

    Deposit AgreementLicense

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    12863_2020_Article_925.pdf
    Size:
    1.704Mb
    Format:
    PDF
    Download
    Author
    Mallikarjunappa, Sanjay
    Shandilya, Umesh K
    Sharma, Ankita
    Lamers, Kristen
    Bissonnette, Nathalie
    Karrow, Niel A
    Meade, Kieran G
    Keyword
    Johne’s disease (JD)
    Interleukin-10 receptor alpha gene (IL10RA)
    CRISPR/cas9
    Gene knockout
    Candidate gene
    Date
    2020-11-02
    
    Metadata
    Show full item record
    Statistics
    Display Item Statistics
    URI
    https://doi.org/10.1186/s12863-020-00925-4; http://hdl.handle.net/11019/2731
    Citation
    Mallikarjunappa, S., Shandilya, U.K., Sharma, A. et al. Functional analysis of bovine interleukin-10 receptor alpha in response to Mycobacterium avium subsp. paratuberculosis lysate using CRISPR/Cas9. BMC Genet 21, 121 (2020). https://doi.org/10.1186/s12863-020-00925-4
    Abstract
    Background The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne’s disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD. The aim of this study was to better understand the functional significance of IL10RA in the context of immune stimulation with MAP cell wall lysate. Results An IL10RA knock out (KO) bovine mammary epithelial cell (MAC-T) line was generated using the CRISPR/cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9) gene editing system. These IL10RA KO cells were stimulated with the immune stimulant MAP lysate +/− IL-10, or with LPS as a positive control. In comparison to unedited cells, relative quantification of immune-related genes after stimulation revealed that knocking out IL10RA resulted in upregulation of pro-inflammatory cytokine gene expression (TNFA, IL1A, IL1B and IL6) and downregulation of suppressor of cytokine signaling 3 (SOCS3), a negative regulator of pro-inflammatory cytokine signaling. At the protein level knocking out IL10RA also resulted in upregulation of inflammatory cytokines - TNF-α and IL-6 and chemokines - IL-8, CCL2 and CCL4, relative to unedited cells. Conclusions The findings of this study illustrate the broad and significant effects of knocking out the IL10RA gene in enhancing pro-inflammatory cytokine expression and further support the immunoregulatory role of IL10RA in eliciting an anti-inflammatory response as well as its potential functional involvement during the immune response associated with JD.
    Funder
    Teagasc Walsh Fellowship Programme; Natural Sciences and Engineering Research Council of Canada (NSERC); Semex Alliance (Canada)
    ae974a485f413a2113503eed53cd6c53
    https://doi.org/10.1186/s12863-020-00925-4
    Scopus Count
    Collections
    Teagasc publications in Biomed Central

    entitlement

     
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.