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dc.contributor.authorByrne, Eoin
dc.contributor.authorBjörkmalm, Johanna
dc.contributor.authorBostick, James P.
dc.contributor.authorSreenivas, Krishnan
dc.contributor.authorWillquist, Karin
dc.contributor.authorvan Niel, Ed W. J.
dc.date.accessioned2021-12-22T15:19:47Z
dc.date.available2021-12-22T15:19:47Z
dc.date.issued2021-10-30
dc.identifier.citationByrne, E., Björkmalm, J., Bostick, J.P. et al. Characterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysates. Biotechnol Biofuels 14, 210 (2021). https://doi.org/10.1186/s13068-021-02058-xen_US
dc.identifier.urihttp://hdl.handle.net/11019/2739
dc.description.abstractAbstract Background The members of the genus Caldicellulosiruptor have the potential for future integration into a biorefinery system due to their capacity to generate hydrogen close to the theoretical limit of 4 mol H2/mol hexose, use a wide range of sugars and can grow on numerous lignocellulose hydrolysates. However, members of this genus are unable to survive in high sugar concentrations, limiting their ability to grow on more concentrated hydrolysates, thus impeding their industrial applicability. In this study five members of this genus, C. owensensis, C. kronotskyensis, C. bescii, C. acetigenus and C. kristjanssonii, were developed to tolerate higher sugar concentrations through an adaptive laboratory evolution (ALE) process. The developed mixed population C. owensensis CO80 was further studied and accompanied by the development of a kinetic model based on Monod kinetics to quantitatively compare it with the parental strain. Results Mixed populations of Caldicellulosiruptor tolerant to higher glucose concentrations were obtained with C. owensensis adapted to grow up to 80 g/L glucose; other strains in particular C. kristjanssonii demonstrated a greater restriction to adaptation. The C. owensensis CO80 mixed population was further studied and demonstrated the ability to grow in glucose concentrations up to 80 g/L glucose, but with reduced volumetric hydrogen productivities ( $$Q_{{{\text{H}}_{2} }}$$ Q H 2 ) and incomplete sugar conversion at elevated glucose concentrations. In addition, the carbon yield decreased with elevated concentrations of glucose. The ability of the mixed population C. owensensis CO80 to grow in high glucose concentrations was further described with a kinetic growth model, which revealed that the critical sugar concentration of the cells increased fourfold when cultivated at higher concentrations. When co-cultured with the adapted C. saccharolyticus G5 mixed culture at a hydraulic retention time (HRT) of 20 h, C. owensensis constituted only 0.09–1.58% of the population in suspension. Conclusions The adaptation of members of the Caldicellulosiruptor genus to higher sugar concentrations established that the ability to develop improved strains via ALE is species dependent, with C. owensensis adapted to grow on 80 g/L, whereas C. kristjanssonii could only be adapted to 30 g/L glucose. Although C. owensensis CO80 was adapted to a higher sugar concentration, this mixed population demonstrated reduced $$Q_{{{\text{H}}_{2} }}$$ Q H 2 with elevated glucose concentrations. This would indicate that while ALE permits adaptation to elevated sugar concentrations, this approach does not result in improved fermentation performances at these higher sugar concentrations. Moreover, the observation that planktonic mixed culture of CO80 was outcompeted by an adapted C. saccharolyticus, when co-cultivated in continuous mode, indicates that the robustness of CO80 mixed culture should be improved for industrial application.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesBiotechnology for Biofuels;
dc.subjectOsmolarityen_US
dc.subjectCaldicellulosiruptoren_US
dc.subjectBiohydrogenen_US
dc.subjectKinetic modelen_US
dc.subjectAdaptive laboratory evolutionen_US
dc.titleCharacterization and adaptation of Caldicellulosiruptor strains to higher sugar concentrations, targeting enhanced hydrogen production from lignocellulosic hydrolysatesen_US
dc.typeArticleen_US
dc.date.updated2021-10-31T04:20:35Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.identifier.doihttps://doi.org/10.1186/s13068-021-02058-x
dc.contributor.sponsorSwedish Energy Agencyen_US
dc.contributor.sponsorFormasen_US
dc.contributor.sponsorVinnovaen_US
dc.contributor.sponsorGrantNumber31090-2en_US
dc.contributor.sponsorGrantNumber2017-00795en_US
dc.contributor.sponsorGrantNumber2017-03286en_US
refterms.dateFOA2021-12-22T15:19:48Z


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