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dc.contributor.authorNiedziela, Dagmara A.
dc.contributor.authorCormican, Paul
dc.contributor.authorFoucras, Gilles
dc.contributor.authorLeonard, Finola C.
dc.contributor.authorKeane, Orla
dc.date.accessioned2021-12-22T15:36:45Z
dc.date.available2021-12-22T15:36:45Z
dc.date.issued2021-11-05
dc.identifier.citationNiedziela, D.A., Cormican, P., Foucras, G. et al. Bovine milk somatic cell transcriptomic response to Staphylococcus aureus is dependent on strain genotype. BMC Genomics 22, 796 (2021). https://doi.org/10.1186/s12864-021-08135-7en_US
dc.identifier.urihttp://hdl.handle.net/11019/2741
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground Mastitis is an economically important disease of dairy cows with Staphylococcus aureus a major cause worldwide. Challenge of Holstein-Friesian cows demonstrated that S. aureus strain MOK124, which belongs to Clonal Complex (CC)151, caused clinical mastitis, while strain MOK023, belonging to CC97, caused mild or subclinical mastitis. The aim of this study was to elucidate the molecular mechanisms of the host immune response utilising a transcriptomic approach. Milk somatic cells were collected from cows infected with either S. aureus MOK023 or MOK124 at 0, 24, 48, 72 and 168 h post-infection (hpi) and analysed for differentially expressed (DE) genes in response to each strain. Results In response to MOK023, 1278, 2278, 1986 and 1750 DE genes were found at 24, 48, 72 and 168 hpi, respectively, while 2293, 1979, 1428 and 1544 DE genes were found in response to MOK124 at those time points. Genes involved in milk production (CSN1, CSN10, CSN1S2, CSN2, a-LACTA and PRLR) were downregulated in response to both strains, with a more pronounced decrease in the MOK124 group. Immune response pathways such as NF-κB and TNF signalling were overrepresented in response to both strains at 24 hpi. These immune pathways continued to be overrepresented in the MOK023 group at 48 and 72 hpi, while the Hippo signalling, extracellular matrix interaction (ECM) and tight junction pathways were overrepresented in the MOK124 group between 48 and 168 hpi. Cellular composition analysis demonstrated that a neutrophil response was predominant in response to MOK124, while M1 macrophages were the main milk cell type post-infection in the MOK023 group. Conclusions A switch from immune response pathways to pathways involved in maintaining the integrity of the epithelial cell layer was observed in the MOK124 group from 48 hpi, which coincided with the occurrence of clinical signs in the infected animals. The higher proportion of M1 macrophages in the MOK023 group and lack of substantial neutrophil recruitment in response to MOK023 may indicate immune evasion by this strain. The results of this study highlight that the somatic cell transcriptomic response to S. aureus is dependent on the genotype of the infecting strain.en_US
dc.language.isoenen_US
dc.publisherBiomed Centralen_US
dc.relation.ispartofseriesBMC Genomics;
dc.subjectMastitisen_US
dc.subjectTranscriptomicsen_US
dc.subjectStaphylococcus aureusen_US
dc.subjectBovine immune responseen_US
dc.subjectRNA-sequencingen_US
dc.subjectHost-pathogen interactionen_US
dc.titleBovine milk somatic cell transcriptomic response to Staphylococcus aureus is dependent on strain genotypeen_US
dc.typeArticleen_US
dc.date.updated2021-11-07T04:14:39Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dc.identifier.doihttps://doi.org/10.1186/s12864-021-08135-7
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorGrantNumber14/S/802en_US
refterms.dateFOA2021-12-22T15:36:45Z


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