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dc.contributor.authorReid, Rachael
dc.contributor.authorBurgess, Catherine M.
dc.contributor.authorMcCabe, Evonne
dc.contributor.authorFanning, Séamus
dc.contributor.authorWhyte, Paul
dc.contributor.authorKerry, Joe
dc.contributor.authorBolton, Declan
dc.date.accessioned2022-12-01T15:43:45Z
dc.date.available2022-12-01T15:43:45Z
dc.date.issued2017-11
dc.identifier.citationRachael Reid, Catherine M. Burgess, Evonne McCabe, Séamus Fanning, Paul Whyte, Joe Kerry, Declan Bolton, Real-time PCR methods for the detection of blown pack spoilage causing Clostridium species; C. estertheticum, C. gasigenes and C. ruminantium, Meat Science, Volume 133, 2017, Pages 56-60, ISSN 0309-1740, https://doi.org/10.1016/j.meatsci.2017.06.001.en_US
dc.identifier.urihttp://hdl.handle.net/11019/2881
dc.descriptionpeer-revieweden_US
dc.description.abstractA set of real-time PCR methods for the detection of C. estertheticum, C. gasigenes and C. ruminantium, the causative agents of blown pack spoilage (BPS) in vacuum packaged beef, was developed. Robust validation of the sensitivity and specificity was carried out in the three matrices (beef meat drip, wet environmental swabs and dry environmental swabs) as encountered in our testing laboratory and against Clostridium strains (n = 76) and non-Clostridium strains (n = 36). It was possible to detect 4–5 spores per ml for C. estertheticum, 2 spores per ml for C. gasigenes and 8 spores per ml for C. ruminantium, without the need for enrichment of the samples. This high sensitivity is particularly important for the beef sector, not just for testing spoiled product but also in the early detection of contaminated beef and in validation of sporicidal cleaning procedures for critical pieces of equipment such as the vacuum packaging machine, which have the potential to contaminate large volumes of product.en_US
dc.description.sponsorshipFood Institutional Research Measure
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesMeat Science;Vol 133
dc.rights© 2017 Elsevier Ltd. All rights reserved.
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectReal-time PCRen_US
dc.subjectBlown pack spoilageen_US
dc.subjectClostridium estertheticumen_US
dc.subjectClostridium gasigenesen_US
dc.subjectClostridium ruminantiumen_US
dc.titleReal-time PCR methods for the detection of blown pack spoilage causing Clostridium species; C. estertheticum, C. gasigenes and C. ruminantiumen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1016/j.meatsci.2017.06.001
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine (Ireland)en_US
dc.contributor.sponsorTeagasc core fundingen_US
dc.source.volume133
dc.source.beginpage56
dc.source.endpage60
refterms.dateFOA2022-12-01T15:43:46Z
dc.source.journaltitleMeat Science


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