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dc.contributor.authorYang, Bo
dc.contributor.authorChen, Haiqin
dc.contributor.authorStanton, Catherine
dc.contributor.authorChen, Yong Q.
dc.contributor.authorZhang, Hao
dc.contributor.authorChen, Wei
dc.date.accessioned2023-02-27T14:36:48Z
dc.date.available2023-02-27T14:36:48Z
dc.date.issued2016-09-22
dc.identifier.citationBo Yang, Haiqin Chen, Catherine Stanton, Yong Q. Chen, Hao Zhang & Wei Chen (2017) Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production, Bioengineered, 8:3, 232-238, DOI: 10.1080/21655979.2016.1222996en_US
dc.identifier.urihttp://hdl.handle.net/11019/2897
dc.descriptionpeer-revieweden_US
dc.description.abstractConjugated linolenic acid (CLNA) is a family of isomers of linolenic acid with a number of healthassociated benefits, which has been attracting great interest. Microbial CLNA producers are potentially an alternative source of CLNA for human nutrition. In present study, 16 neonate feces were collected and used for Bifidobacteria isolation, from which 25 bifidobacteria isolates were obtained. The bifidobacteria isolates were identified using 16s rDNA sequencing as Bifidobacterium adolescentis, B. breve, B. longum and B. pseudocatenulatum. These isolates were further investigated for their ability to produce CLNA using linolenic acid as substrate via GC-MS. The results showed most of the isolates could convert free linolenic acid into c9,t11,c15-CLNA and t9,t11,c15-CLNA at different levels. B. pseudocatenulatum was the most effective CLNA producer, which converted 86.91% of linolenic acid to c9,t11,c15-CLNA and 3.59% of to t9,t11,c15-CLNA isomer and the isolate exhibited to accumulate CLNA during 72 h culturing in which most CLNA isomers were in the supernatant fluid. The results indicated that utilization of this isolate for CLNA production will eliminate the purification process.en_US
dc.description.sponsorshipNational Natural Science Foundation of China
dc.language.isoenen_US
dc.publisherInforma UK Limiteden_US
dc.relation.ispartofseriesBioengineered;Vol 8
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectbifidobacteriaen_US
dc.subjectbioconversionen_US
dc.subjectconjugated linolenic aciden_US
dc.subjectisolationen_US
dc.titleMining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid productionen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1080/21655979.2016.1222996
dc.contributor.sponsorNational Natural Science Foundation of Chinaen_US
dc.contributor.sponsorthe Program for New Century Excellent Talentsen_US
dc.contributor.sponsorthe Program for Changjiang Scholars and Innovative Research Team in Universityen_US
dc.contributor.sponsorthe National Natural Science Foundation of Jiangsu Provinceen_US
dc.contributor.sponsorthe Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control.”en_US
dc.contributor.sponsorGrantNumberNos. 31571810, 31530056en_US
dc.contributor.sponsorGrantNumberNCET-13-0831en_US
dc.contributor.sponsorGrantNumberIRT1249en_US
dc.contributor.sponsorGrantNumberBK20150141en_US
dc.source.volume8
dc.source.issue3
dc.source.beginpage232
dc.source.endpage238
refterms.dateFOA2023-02-27T14:36:49Z
dc.source.journaltitleBioengineered


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