Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production
dc.contributor.author | Yang, Bo | |
dc.contributor.author | Chen, Haiqin | |
dc.contributor.author | Stanton, Catherine | |
dc.contributor.author | Chen, Yong Q. | |
dc.contributor.author | Zhang, Hao | |
dc.contributor.author | Chen, Wei | |
dc.date.accessioned | 2023-02-27T14:36:48Z | |
dc.date.available | 2023-02-27T14:36:48Z | |
dc.date.issued | 2016-09-22 | |
dc.identifier.citation | Bo Yang, Haiqin Chen, Catherine Stanton, Yong Q. Chen, Hao Zhang & Wei Chen (2017) Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production, Bioengineered, 8:3, 232-238, DOI: 10.1080/21655979.2016.1222996 | en_US |
dc.identifier.uri | http://hdl.handle.net/11019/2897 | |
dc.description | peer-reviewed | en_US |
dc.description.abstract | Conjugated linolenic acid (CLNA) is a family of isomers of linolenic acid with a number of healthassociated benefits, which has been attracting great interest. Microbial CLNA producers are potentially an alternative source of CLNA for human nutrition. In present study, 16 neonate feces were collected and used for Bifidobacteria isolation, from which 25 bifidobacteria isolates were obtained. The bifidobacteria isolates were identified using 16s rDNA sequencing as Bifidobacterium adolescentis, B. breve, B. longum and B. pseudocatenulatum. These isolates were further investigated for their ability to produce CLNA using linolenic acid as substrate via GC-MS. The results showed most of the isolates could convert free linolenic acid into c9,t11,c15-CLNA and t9,t11,c15-CLNA at different levels. B. pseudocatenulatum was the most effective CLNA producer, which converted 86.91% of linolenic acid to c9,t11,c15-CLNA and 3.59% of to t9,t11,c15-CLNA isomer and the isolate exhibited to accumulate CLNA during 72 h culturing in which most CLNA isomers were in the supernatant fluid. The results indicated that utilization of this isolate for CLNA production will eliminate the purification process. | en_US |
dc.description.sponsorship | National Natural Science Foundation of China | |
dc.language.iso | en | en_US |
dc.publisher | Informa UK Limited | en_US |
dc.relation.ispartofseries | Bioengineered;Vol 8 | |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.subject | bifidobacteria | en_US |
dc.subject | bioconversion | en_US |
dc.subject | conjugated linolenic acid | en_US |
dc.subject | isolation | en_US |
dc.title | Mining bifidobacteria from the neonatal gastrointestinal tract for conjugated linolenic acid production | en_US |
dc.type | Article | en_US |
dc.identifier.doi | https://doi.org/10.1080/21655979.2016.1222996 | |
dc.contributor.sponsor | National Natural Science Foundation of China | en_US |
dc.contributor.sponsor | the Program for New Century Excellent Talents | en_US |
dc.contributor.sponsor | the Program for Changjiang Scholars and Innovative Research Team in University | en_US |
dc.contributor.sponsor | the National Natural Science Foundation of Jiangsu Province | en_US |
dc.contributor.sponsor | the Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control.” | en_US |
dc.contributor.sponsorGrantNumber | Nos. 31571810, 31530056 | en_US |
dc.contributor.sponsorGrantNumber | NCET-13-0831 | en_US |
dc.contributor.sponsorGrantNumber | IRT1249 | en_US |
dc.contributor.sponsorGrantNumber | BK20150141 | en_US |
dc.source.volume | 8 | |
dc.source.issue | 3 | |
dc.source.beginpage | 232 | |
dc.source.endpage | 238 | |
refterms.dateFOA | 2023-02-27T14:36:49Z | |
dc.source.journaltitle | Bioengineered |
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