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dc.contributor.authorHowick, Ken
dc.contributor.authorWallace-Fitzsimons, Shauna
dc.contributor.authorKandil, Dalia
dc.contributor.authorChruścicka, Barbara
dc.contributor.authorCalis, Mert
dc.contributor.authorMurphy, Eoin
dc.contributor.authorMurray, Brian
dc.contributor.authorFernandez, Ayoa
dc.contributor.authorBarry, Kate
dc.contributor.authorKelly, Phil
dc.contributor.authorRyan, Aoife
dc.contributor.authorCryan, John
dc.contributor.authorGriffin, Brendan
dc.contributor.authorSchellekens, Harriët
dc.date.accessioned2023-06-26T09:02:35Z
dc.date.available2023-06-26T09:02:35Z
dc.date.issued2018-09-15
dc.identifier.citationHowick, K.; Wallace-Fitzsimons, S.E.; Kandil, D.; Chruścicka, B.; Calis, M.; Murphy, E.; Murray, B.A.; Fernandez, A.; Barry, K.M.; Kelly, P.M.; Ryan, A.M.; Cryan, J.F.; Griffin, B.T.; Schellekens, H. A Dairy-Derived Ghrelinergic Hydrolysate Modulates Food Intake In Vivo. Int. J. Mol. Sci. 2018, 19, 2780. https://doi.org/10.3390/ijms19092780en_US
dc.identifier.urihttp://hdl.handle.net/11019/2943
dc.descriptionpeer-revieweden_US
dc.description.abstractRecent times have seen an increasing move towards harnessing the health-promoting benefits of food and dietary constituents while providing scientific evidence to substantiate their claims. In particular, the potential for bioactive protein hydrolysates and peptides to enhance health in conjunction with conventional pharmaceutical therapy is being investigated. Dairy-derived proteins have been shown to contain bioactive peptide sequences with various purported health benefits, with effects ranging from the digestive system to cardiovascular circulation, the immune system and the central nervous system. Interestingly, the ability of dairy proteins to modulate metabolism and appetite has recently been reported. The ghrelin receptor (GHSR-1a) is a G-protein coupled receptor which plays a key role in the regulation of food intake. Pharmacological manipulation of the growth hormone secretagogue receptor-type 1a (GHSR-1a) receptor has therefore received a lot of attention as a strategy to combat disorders of appetite and body weight, including age-related malnutrition and the progressive muscle wasting syndrome known as cachexia. In this study, a milk protein-derivative is shown to increase GHSR-1a-mediated intracellular calcium signalling in a concentration-dependent manner in vitro. Significant increases in calcium mobilisation were also observed in a cultured neuronal cell line heterologously expressing the GHS-R1a. In addition, both additive and synergistic effects were observed following co-exposure of GHSR-1a to both the hydrolysate and ghrelin. Subsequent in vivo studies monitored standard chow intake in healthy male and female Sprague-Dawley rats after dosing with the casein hydrolysate (CasHyd). Furthermore, the provision of gastro-protected oral delivery to the bioactive in vivo may aid in the progression of in vitro efficacy to in vivo functionality. In summary, this study reports a ghrelin-stimulating bioactive peptide mixture (CasHyd) with potent effects in vitro. It also provides novel and valuable translational data supporting the potential role of CasHyd as an appetite-enhancing bioactive. Further mechanistic studies are required in order to confirm efficacy as a ghrelinergic bioactive in susceptible population groups.en_US
dc.language.isoenen_US
dc.publisherMultidisciplinary Digital Publishing Instituteen_US
dc.relation.ispartofseriesInternational Journal of Molecular Sciences;Vol 19
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectappetiteen_US
dc.subjectbioactive peptidesen_US
dc.subjectcachexiaen_US
dc.subjectcalcium mobilisationen_US
dc.subjectdairyen_US
dc.subjectfood intakeen_US
dc.subjectghrelinen_US
dc.subjectghrelin receptoren_US
dc.subjectmalnutritionen_US
dc.titleA Dairy-Derived Ghrelinergic Hydrolysate Modulates Food Intake In Vivoen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3390/ijms19092780
dc.contributor.sponsorEnterprise Irelanden_US
dc.contributor.sponsorGrantNumberTC20130001en_US
dc.source.volume19
dc.source.issue9
dc.source.beginpage2780
refterms.dateFOA2023-06-26T09:02:36Z
dc.source.journaltitleInternational Journal of Molecular Sciences


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