Show simple item record

dc.contributor.authorJohnston, Daniel G W
dc.contributor.authorWilliams, Michelle A
dc.contributor.authorThaiss, Christoph A
dc.contributor.authorCabrera-Rubio, Raul
dc.contributor.authorRaverdeau, Mathilde
dc.contributor.authorMcEntee, Craig
dc.contributor.authorCotter, Paul D
dc.contributor.authorElinav, Eran
dc.contributor.authorO’Neill, Luke A J
dc.contributor.authorCorr, Sinéad C
dc.date.accessioned2023-06-26T15:20:14Z
dc.date.available2023-06-26T15:20:14Z
dc.date.issued2018-03-28
dc.identifier.citationDaniel G W Johnston, Michelle A Williams, Christoph A Thaiss, Raul Cabrera-Rubio, Mathilde Raverdeau, Craig McEntee, Paul D Cotter, Eran Elinav, Luke A J O’Neill, Sinéad C Corr, Loss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitis, Journal of Crohn's and Colitis, Volume 12, Issue 7, July 2018, Pages 835–848, https://doi.org/10.1093/ecco-jcc/jjy038en_US
dc.identifier.urihttp://hdl.handle.net/11019/2946
dc.descriptionpeer-revieweden_US
dc.description.abstractBackground and Aims microRNAs regulate gene expression and influence the pathogenesis of human diseases. The present study investigated the role of microRNA-21 [miR-21] in the pathogenesis of intestinal inflammation, because miR-21 is highly expressed in inflammatory bowel disease. Inflammatory bowel disease is associated with intestinal barrier dysfunction and an altered gut microbiota. Recent studies have demonstrated that host microRNAs can shape the microbiota. Thus, we determined the influence of miR-21 on the gut microbiota and observed the subsequent impact in a dextran sodium sulphate [DSS]-induced colitis model. Methods The influence of miR-21 on the gut microbiota and inflammation was assessed in wild-type [WT] and miR-21–/– mice, in co-housed mice, following antibiotic depletion of the microbiota, or by colonization of germ-free [GF] mice with fecal homogenate, prior to DSS administration. We carried out 16S rRNA sequencing on WT and miR-21–/– mice to dissect potential differences in the gut microbiota. Results miR-21–/– mice have reduced susceptibility to DSS-induced colitis compared with WT mice. Co-housing conferred some protection to WT mice, while GF mice colonized with fecal homogenate from miR-21–/– were protected from DSS colitis compared with those colonized with WT homogenate. Further supporting a role for the microbiota in the observed phenotype, the protection afforded by miR-21 depletion was lost when mice were pre-treated with antibiotics. The 16S rRNA sequencing revealed significant differences in the composition of WT and miR-21–/– intestinal microbiota. Conclusions These findings suggest that miR-21 influences the pathogenesis of intestinal inflammation by causing propagation of a disrupted gut microbiota.en_US
dc.description.sponsorshipScience Foundation Ireland
dc.language.isoenen_US
dc.publisherOxford University Press (OUP)en_US
dc.relation.ispartofseriesJournal of Crohn's and Colitis;Vol 12
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttps://academic.oup.com/journals/pages/about_us/legal/notices
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectMicroRNAen_US
dc.subjectmicrobiotaen_US
dc.subjectinflammationen_US
dc.titleLoss of MicroRNA-21 Influences the Gut Microbiota, Causing Reduced Susceptibility in a Murine Model of Colitisen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.1093/ecco-jcc/jjy038
dc.contributor.sponsorScience Foundation Ireland [SFI]en_US
dc.contributor.sponsorGrantNumber11/SIRG/B2099en_US
dc.source.volume12
dc.source.issue7
dc.source.beginpage835
dc.source.endpage848
refterms.dateFOA2023-06-26T15:20:17Z
dc.source.journaltitleJournal of Crohn's and Colitis


Files in this item

Thumbnail
Name:
jjy038.pdf
Size:
2.114Mb
Format:
PDF
Description:
main article

This item appears in the following Collection(s)

Show simple item record