Draft Genome Sequences of Four Lactococcus lactis Strains Isolated from Diverse Niches, Including Dairy Products, Grass, and Green Peas
Lactococcus lactis subsp. cremoris DPC 140
Lactococcus lactis subsp. cremoris DPC 169
Lactococcus lactis subsp. lactis DPC 6756
Lactococcus lactis subsp. cremoris DPC 6855
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CitationRoman Naranjo D, Callanan M, McAuliffe O. Draft Genome Sequences of Four Lactococcus lactis Strains Isolated from Diverse Niches, Including Dairy Products, Grass, and Green Peas. Microbiol Resour Announc. 2019 Aug 29;8(35):e00834-19. doi: 10.1128/MRA.00834-19. PMID: 31467105; PMCID: PMC6715875.
AbstractLactococcus lactis has been used for millennia as a starter organism in the production of many fermented dairy products. This announcement includes the draft genome sequences of four strains of Lactococcus lactis, two of dairy origin and two from nondairy sources.
FunderDairy Research Ireland; Teagasc Walsh Fellowship Program
Grant Number0402; 2018036
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Lactococcus lactis subsp. lactis as a natural anti-listerial agent in the mushroom industryDygico, Lionel K.; O'Connor, Paula M.; Hayes, Maria; Gahan, Cormac G M; Grogan, Helen; Burgess, Catherine; Department of Agriculture, Food & the Marine; 14F881 (Elsevier, 2019-01-28)Mushroom growth substrates from different commercial producers of mushrooms (Agaricus bisporus) were screened for the presence of bacteria with potential for use as biocontrol agents for controlling Listeria monocytogenes in the mushroom production environment. Eight anti-listerial strains were isolated from different sources and all were identified using 16s rRNA gene sequencing as Lactococcus lactis subsp. lactis. Whole-genome sequencing of the Lc. lactis isolates indicated that strains from different sites and substrate types were highly similar. Colony MALDI-TOF mass spectrometry found that these strains were Nisin Z producers but inhibitory activity was highly influenced by the incubation conditions and was strain dependant. The biofilm forming ability of these strains was tested using a crystal violet assay and all were found to be strong biofilm formers. Growth of Lc. lactis subsp. lactis using mixed-biofilm conditions with L. monocytogenes on stainless steel resulted in a 4-log reduction of L. monocytogenes cell numbers. Additional sampling of mushroom producers showed that these anti-listerial Lc. lactis strains are commonly present in the mushroom production environment. Lc. lactis has a generally regarded as safe (GRAS) status and therefore has potential for use as an environmentally benign solution to control L. monocytogenes in order to prevent product contamination and to enhance consumer confidence in the mushroom industry.
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