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dc.contributor.authorQuigley, Lisa
dc.contributor.authorCoakley, Mairéad
dc.contributor.authorAlemayehu, Debebe
dc.contributor.authorRea, Mary C.
dc.contributor.authorCasey, Patrick G.
dc.contributor.authorO’Sullivan, Órla
dc.contributor.authorMurphy, Eileen
dc.contributor.authorKiely, Barry
dc.contributor.authorCotter, Paul D.
dc.contributor.authorHill, Colin
dc.contributor.authorRoss, R. Paul
dc.identifier.citationQuigley L, Coakley M, Alemayehu D, Rea MC, Casey PG, O’Sullivan Ó, Murphy E, Kiely B, Cotter PD, Hill C and Ross RP (2019) Lactobacillus gasseri APC 678 Reduces Shedding of the Pathogen Clostridium difficile in a Murine Model. Front. Microbiol. 10:273. doi: 10.3389/fmicb.2019.00273en_US
dc.description.abstractClostridium difficile is a common cause of health-care acquired diarrhea, resulting in a spectrum of disease from mild diarrhea to life-threatening illness. Sixty Lactobacillus strains were screened for anti-C. difficile activity using a co-culture method. Based on their ability to inhibit C. difficile, L. gasseri APC 678 and L. rhamnosus DPC 6111 were selected for study in a murine model of C. difficile infection. L. gasseri ATCC 33323, was included as a control. It was established that, relative to control mice not fed Lactobacillus, feeding with L. gasseri APC 678 resulted in a significant reduction by day 7 (8-fold, p = 0.017) of viable C. difficile VPI 10463 in the feces of mice. In contrast, neither L. rhamnosus DPC 6111 nor L. gasseri ATCC 33323 significantly reduced fecal C. difficile shedding. Sequencing of the cecal microbiota showed that in mice fed L. gasseri APC 678 there was a significant increase in bacterial diversity across a number of indices when compared to the control or other Lactobacillus-fed groups. There was no significant change in the relative abundance of Firmicutes or Bacteroidetes in the group fed L. gasseri APC 678 relative to the control, while the groups fed L. rhamnosus DPC 6111 or L. gasseri ATCC 33323 showed a significant decrease in the relative abundance of Firmicutes (p = 0.002 and p = 0.019, respectively) and a significant increase in Bacteroidetes (p = 0.002 and p = 0.023, respectively). These results highlight the potential of L. gasseri APC 678 as a live therapeutic agent to target C. difficile infection.en_US
dc.description.sponsorshipScience Foundation Ireland
dc.publisherFrontiers Media SAen_US
dc.relation.ispartofseriesFrontiers in Microbiology;Vol 10
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectLactobacillus gasserien_US
dc.subjectClostridium difficileen_US
dc.subjectC. difficile infection (CDI)en_US
dc.subjectmurine modelen_US
dc.subjectlive therapeutic agenten_US
dc.titleLactobacillus gasseri APC 678 Reduces Shedding of the Pathogen Clostridium difficile in a Murine Modelen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorAlimentary Health Ltden_US
dc.source.journaltitleFrontiers in Microbiology

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