Show simple item record

dc.contributor.authorDobbs, Edward
dc.contributor.authorDeakin, Greg
dc.contributor.authorBennett, Julie
dc.contributor.authorFleming-Archibald, Caoimhe
dc.contributor.authorJones, Ian
dc.contributor.authorGrogan, Helen
dc.contributor.authorBurton, Kelly
dc.identifier.citationDobbs E, Deakin G, Bennett J, Fleming-Archibald C, Jones I, Grogan H, Burton K. 2021. Viral interactions and pathogenesis during multiple viral infections in Agaricus bisporus. mBio 12:e03470-20. .1128/mBio.03470-20en_US
dc.description.abstractViral interactions during multiple viral infections were examined in Agaricus bisporus cultures harboring 9 viruses (comprising 18 distinct viral RNAs) by statistically analyzing their relative abundance in fruitbodies. Four clusters of viral RNA were identified that suggested synergism and coreplication. Pairwise correlations revealed negative and positive correlations between clusters, indicating further synergisms and an antagonism involving a group containing a putative hypovirus and four nonhost ORFan RNAs (RNAs with no similarity to known sequences) possibly acting as defective interfering RNAs. The disease phenotype was observed in 10 to 15% of the fruitbodies apparently randomly located among asymptomatic fruitbodies. The degree of symptom expression consistently correlated with the levels of the multipartite virus AbV16. Diseased fruitbodies contained very high levels of AbV16 and AbV6 RNA2; these levels were orders of magnitude higher than those in asymptomatic tissues and were shown statistically to be discretely higher populations of abundance, indicating an exponential shift in the replicative capacity of the virus. High levels of AbV16 replication were specific to the fruitbody and not found in the underlying mycelium. There appeared to be a stochastic element occurring in these viral interactions, as observed in the distribution of diseased symptoms across a culture, differences in variance between experiments, and a number of additional viruses undergoing the step-jump in levels between experiments. Possible mechanisms for these multiple and simultaneous viral interactions in single culture are discussed in relation to known virus-host regulatory mechanisms for viral replication and whether additional factors could be considered to account for the 1,000-fold increase in AbV16 and AbV6 RNA2 levelsen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofseriesmBio;Vol 12
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectAgaricus bisporusen_US
dc.subjectmultiple-virus infectionen_US
dc.subjectplus-strand RNA virusen_US
dc.titleViral Interactions and Pathogenesis during Multiple Viral Infections in Agaricus bisporusen_US
dc.contributor.sponsorTeagasc Walsh Fellowship Programen_US
dc.contributor.sponsorEuropean Union Seventh Framework Programen_US
dc.contributor.sponsorGrantNumberproject 201043en_US

Files in this item

main article

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-ShareAlike 4.0 International
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International