Localization of urea transporter B in the developing bovine rumen
Waters, Sinead M.
Stewart, Gavin S.
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CitationChongliang Zhong, Tamsin Lyons, Orla Heussaff, Evelyn Doyle, Eoin O'Hara, Sinead M. Waters, David Kenny, Gavin S. Stewart, Localization of urea transporter B in the developing bovine rumen, Animal Nutrition, Volume 10, 2022, Pages 216-222, ISSN 2405-6545, https://doi.org/10.1016/j.aninu.2022.03.006.
AbstractUrea nitrogen secreted from blood to rumen is a crucial factor shaping the symbiotic relationship between host ruminants and their microbial populations. Passage of urea across rumen epithelia is facilitated by urea transporter B (UT-B), but the long-term regulation of these proteins remains unclear. As ruminal function develops over a period of months, the developing rumen is an excellent model with which to investigate this regulation. Using rumen epithelium samples of calves from birth to 96 d of age, this study performed immunolocalization studies to localize and semi-quantify UT-B protein development. As expected, preliminary experiments confirmed that ruminal monocarboxylate transporter 1 (MCT1) short chain fatty acid transporter protein abundance increased with age (P < 0.01, n = 4). Further investigation revealed that ruminal UT-B was present in the first few weeks of life and initially detected in the basolateral membrane of stratum basale cells. Over the next 2 months, UT-B staining spread to other epithelial layers and semi-quantification indicated that UT-B abundance significantly increased with age (P < 0.01, n = 4 or 6). These changes were in line with the development of rumen function after the advent of solid feed intake and weaning, exhibiting a similar pattern to both MCT1 transporters and papillae growth. This study therefore confirmed age-dependent changes of in situ ruminal UT-B protein, adding to our understanding of the long-term regulation of ruminal urea transporters.
FunderThe China Scholarship Council; University College Dublin
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