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dc.contributor.authorLawrence, Garreth W.
dc.contributor.authorMcCarthy, Niamh
dc.contributor.authorWalsh, Calum J.
dc.contributor.authorKunyoshi, Tais M.
dc.contributor.authorLawton, Elaine M.
dc.contributor.authorO’Connor, Paula M.
dc.contributor.authorBegley, Máire
dc.contributor.authorCotter, Paul D.
dc.contributor.authorGuinane, Caitriona M.
dc.identifier.citationGarreth W. Lawrence, Niamh McCarthy, Calum J. Walsh, Tais M. Kunyoshi, Elaine M. Lawton, Paula M. O’Connor, Máire Begley, Paul D. Cotter & Caitriona M. Guinane (2022) Effect of a bacteriocin-producing Streptococcus salivarius on the pathogen Fusobacterium nucleatum in a model of the human distal colon, Gut Microbes, 14:1, 2100203, DOI: 10.1080/19490976.2022.2100203en_US
dc.description.abstractThe gut microbiome is a vast reservoir of microbes, some of which produce antimicrobial peptides called bacteriocins that may inhibit specific bacteria associated with disease. Fusobacterium nucleatum is an emerging human bacterial pathogen associated with gastrointestinal diseases including colorectal cancer (CRC). In this study, fecal samples of healthy donors were screened for potential bacteriocin-producing probiotics with antimicrobial activity against F. nucleatum. A novel isolate, designated as Streptococcus salivarius DPC6993 demonstrated a narrow-spectrum of antimicrobial activity against F. nucleatum in vitro. In silico analysis of the S. salivarius DPC6993 genome revealed the presence of genes involved in the production of the bacteriocins salivaricin A5 and salivaricin B. After 6 h in a colon fermentation model, there was a significant drop in the number of F. nucleatum in samples that had been simultaneously inoculated with S. salivarius DPC6993 + F. nucleatum DSM15643 compared to those inoculated with F. nucleatum DSM15643 alone (mean ± SD: 9243.3 ± 3408.4 vs 29688.9 ± 4993.9 copies/μl). Furthermore, 16S rRNA amplicon analysis revealed a significant difference in the mean relative abundances of Fusobacterium between samples inoculated with both S. salivarius DPC6993 and F. nucleatum DSM15643 (0.05%) and F. nucleatum DSM15643 only (0.32%). Diversity analysis indicated minimal impact exerted by S. salivarius DPC6993 on the surrounding microbiota. Overall, this study highlights the ability of a natural gut bacterium to target a bacterial pathogen associated with CRC. The specific targeting of CRC-associated pathogens by biotherapeutics may ultimately reduce the risk of CRC development and positively impact CRC outcomes.en_US
dc.publisherInforma UK Limiteden_US
dc.relation.ispartofseriesGut microbes;Vol 14
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectFusobacterium nucleatumen_US
dc.subjectStreptococcus salivariusen_US
dc.subjectbiotherapeutics; probioticsen_US
dc.subjectcolorectal canceren_US
dc.subjectcolon modelen_US
dc.titleEffect of a bacteriocin-producing Streptococcus salivarius on the pathogen Fusobacterium nucleatum in a model of the human distal colonen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorIrish Department of Agriculture, Food and the Marineen_US
dc.contributor.sponsorEnterprise Irelanden_US
dc.contributor.sponsorEuropean Commissionen_US
dc.contributor.sponsorGrantNumber818368 (MASTER)en_US
dc.source.journaltitleGut Microbes

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