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dc.contributor.authorYang, Yuqing
dc.contributor.authorAdela Wasiewska, Luiza
dc.contributor.authorBurgess, Catherine M.
dc.contributor.authorDuffy, Geraldine
dc.contributor.authorLovera, Pierre
dc.contributor.authorO'Riordan, Alan
dc.identifier.citationYuqing Yang, Luiza Adela Wasiewska, Catherine M. Burgess, Geraldine Duffy, Pierre Lovera, Alan O’Riordan, Detection of stx2 from Shiga toxin-producing Escherichia coli (STEC) by a surface enhanced Raman spectroscopy (SERS) sensor using recycled silicon chips, Sensors and Actuators B: Chemical, Volume 373, 2022, 132618, ISSN 0925-4005,
dc.description.abstractIn this research, a selective, cost-efficient, and highly sensitive Ag nanostructure Surface Enhanced Raman Spectroscopy (SERS) sensor was developed as a methodological approach to rapidly detect a targetss-DNA (stx2) in STEC (Shiga toxin-producing Escherichia coli). The Ag nanostructure-based SERS substrate was functionalized by two types of thiols: thiol-ss-DNA for bonding target ss-DNA and 6-Mercapto-1-hexanol (HS(CH2)6OH) for blocking the Ag nanostructure surface. Methylene Blue (MB) was used as a Raman marker to quantify target ss-DNA, as well as a model molecule to characterize the electrodeposited Ag nanostructure SERS substrate. Ag nanostructure SERS substrates showed good sensitivity and repeatability towards MB detection, with a LOD = 0.3158 μM, and RSD = 12.48% (at 45 different random points for 0.1 μM MB). More importantly, the Ag nanostructure/ss-DNA SERS substrate showed good selectivity towards STEC O157 stx2 target DNA, as well as good linearity and sensitivity towards its detection in a buffer solution. A limit of detection of 0.4900 aM and a wide linear range from 1 aM to 100 pM were demonstrated. The SERS sensors were able to identify target DNA (stx2) in a STEC strain and the study showed proof of principle that SERS substrate has potential as a cost-effective, highly selective, highly sensitive DNA and bacteria sensor without the aid of DNA amplification. With further development and validation, this methodological approach has the potential for point-of-use detection for instance on a farm or in the food industry.en_US
dc.relation.ispartofseriesSensors and Actuators B: Chemical;Vol 373
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectShiga toxin-producing Escherichia coli (E. coli)en_US
dc.subjectDNA detectionen_US
dc.subjectSurface-enhanced Raman spectroscopyen_US
dc.subjectThiol functionalized Ag nanostructure SERS substrateen_US
dc.titleDetection of stx2 from Shiga toxin-producing Escherichia coli (STEC) by a surface enhanced Raman spectroscopy (SERS) sensor using recycled silicon chipsen_US
dc.contributor.sponsorEuropean Unionen_US
dc.contributor.sponsorScience Foundation Irelanden_US
dc.contributor.sponsorDepartment of Agriculture, Food and the Marineen_US
dc.contributor.sponsorTeagasc Walsh Scholarshipen_US

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