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dc.contributor.authorBattersby, Tara
dc.contributor.authorWalsh, Desmond
dc.contributor.authorWhyte, Paul
dc.contributor.authorBolton, Declan J.
dc.identifier.citationTara Battersby , Desmond Walsh , Paul Whyte & Declan J. Bolton (2016) Campylobacter growth rates in four different matrices: broiler caecal material, live birds, Bolton broth, and brain heart infusion broth, Infection Ecology & Epidemiology, 6:1, DOI: 10.3402/iee.v6.31217en_US
dc.description.abstractBackground The objective of this study was to characterise Campylobacter growth in enrichment broths (Bolton broth, brain heart infusion broth), caecal material (in vitro), and in the naturally infected live broilers (in vivo) in terms of mean lag periods and generation times as well as maximum growth rates and population (cell concentration) achieved. Methods Bolton and brain heart infusion broths and recovered caecal material were inoculated with 10 poultry strains of Campylobacter (eight Campylobacter jejuni and two Campylobacter coli), incubated under microaerobic conditions, and Campylobacter concentrations determined periodically using the ISO 10272:2006 method. Caeca from 10 flocks, infected at first thinning, were used to characterise Campylobacter growth in the live birds. Mean generation times (G) (early lag to exponential phase) were calculated using the formula: G=t/3.3 logb/B. Mean lag times and µmax were calculated using the Micro Fit© Software (Version 1.0, Institute of Food Research). Statistical comparison was performed using GENSTAT ver. 14.1 (VSN International Ltd., Hemel, Hempstead, UK). Results The mean lag periods in Bolton broth, brain heart infusion broth, caecal material, and in the live bird were estimated to be 6.6, 6.7, 12.6, and 31.3 h, respectively. The corresponding mean generation times were 2.1, 2.2, 3.1, and 6.7 h, respectively; maximum growth rates were 0.7, 0.8, 0.4, and 2 generations h−1 and the maximum populations obtained in each matrix were 9.6, 9.9, 7.8, and 7.4 log10 CFU/g, respectively. Conclusion This study provides data on the growth of Campylobacter in a range of laboratory media, caecal contents, and in broilers which may be used to develop predictive models and/or inform science-based control strategies such as the maximum time between flock testing and slaughter, logistical slaughter, and single-stage depopulation of broiler units.en_US
dc.publisherInforma UK Limiteden_US
dc.relation.ispartofseriesInfection Ecology & Epidemiology;Vol 6
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.subjectGrowth rates in vivo and in vitroen_US
dc.subjectflock thinningen_US
dc.subjectfoodborne pathogenen_US
dc.titleCampylobacter growth rates in four different matrices: broiler caecal material, live birds, Bolton broth, and brain heart infusion brothen_US
dc.contributor.sponsorDepartment of Agriculture, Food and Marine (Ireland)en_US
dc.contributor.sponsorGrantNumberProject 11/F/051en_US
dc.source.journaltitleInfection Ecology & Epidemiology

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