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    Global Gene Expression and Systems Biology Analysis of Bovine Monocyte-Derived Macrophages in Response to In Vitro Challenge with Mycobacterium bovis

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    Author
    Magee, David A
    Taraktsoglou, Maria
    Killick, Kate E
    Nalpas, Nicolas C.
    Browne, John A
    Park, Stephen D. E.
    Conlon, Kevin M.
    Lynn, David J
    Hokamp, Karsten
    Gordon, Stephen V
    Gormley, Eamonn
    MacHugh, David E
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    Keyword
    Mycobacterium bovis
    bovine tuberculosis
    monocyte-derived macrophages
    global gene expression analysis
    Date
    2012-02-22
    
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    URI
    http://hdl.handle.net/11019/332
    Citation
    Magee DA, Taraktsoglou M, Killick KE, Nalpas NC, Browne JA, et al. (2012) Global Gene Expression and Systems Biology Analysis of Bovine Monocyte-Derived Macrophages in Response to In Vitro Challenge with Mycobacterium bovis. PLoS ONE 7(2): e32034. doi:10.1371/journal.pone.0032034
    Abstract
    Background Mycobacterium bovis, the causative agent of bovine tuberculosis, is a major cause of mortality in global cattle populations. Macrophages are among the first cell types to encounter M. bovis following exposure and the response elicited by these cells is pivotal in determining the outcome of infection. Here, a functional genomics approach was undertaken to investigate global gene expression profiles in bovine monocyte-derived macrophages (MDM) purified from seven age-matched non-related females, in response to in vitro challenge with M. bovis (multiplicity of infection 2:1). Total cellular RNA was extracted from non-challenged control and M. bovis-challenged MDM for all animals at intervals of 2 hours, 6 hours and 24 hours post-challenge and prepared for global gene expression analysis using the Affymetrix® GeneChip® Bovine Genome Array. Results Comparison of M. bovis-challenged MDM gene expression profiles with those from the non-challenged MDM controls at each time point identified 3,064 differentially expressed genes 2 hours post-challenge, with 4,451 and 5,267 differentially expressed genes detected at the 6 hour and 24 hour time points, respectively (adjusted P-value threshold ≤0.05). Notably, the number of downregulated genes exceeded the number of upregulated genes in the M. bovis-challenged MDM across all time points; however, the fold-change in expression for the upregulated genes was markedly higher than that for the downregulated genes. Systems analysis revealed enrichment for genes involved in: (1) the inflammatory response; (2) cell signalling pathways, including Toll-like receptors and intracellular pathogen recognition receptors; and (3) apoptosis. Conclusions The increased number of downregulated genes is consistent with previous studies showing that M. bovis infection is associated with the repression of host gene expression. The results also support roles for MyD88-independent signalling and intracellular PRRs in mediating the host response to M. bovis.
    Funder
    Department of Agriculture, Food and the Marine; European Union; Science Foundation Ireland; Irish Research Council for Science, Engineering and Technology
    Grant Number
    RSF 06 405; KBBE-211602-MACROSYS; SFI/01/F.1/B028; SFI/08/IN.1/B2038
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1371/journal.pone.0032034
    Scopus Count
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    Animal & Bioscience

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