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dc.contributor.authorDrake, E.
dc.contributor.authorHolden, S.A.
dc.contributor.authorAublet, V.
dc.contributor.authorDoyle, R.C.
dc.contributor.authorMillar, C.
dc.contributor.authorMoore, S.G.
dc.contributor.authorMaicas, C.
dc.contributor.authorRandi, F.
dc.contributor.authorCromie, A.R.
dc.contributor.authorLonergan, P.
dc.contributor.authorButler, S.T.
dc.date.accessioned2023-12-19T15:28:21Z
dc.date.available2023-12-19T15:28:21Z
dc.date.issued2020-12
dc.identifier.citationE. Drake, S.A. Holden, V. Aublet, R.C. Doyle, C. Millar, S.G. Moore, C. Maicas, F. Randi, A.R. Cromie, P. Lonergan, S.T. Butler, Evaluation of delayed timing of artificial insemination with sex-sorted sperm on pregnancy per artificial insemination in seasonal-calving, pasture-based lactating dairy cows, Journal of Dairy Science, Volume 103, Issue 12, 2020, Pages 12059-12068, ISSN 0022-0302, https://doi.org/10.3168/jds.2020-18847.en_US
dc.identifier.urihttp://hdl.handle.net/11019/3413
dc.descriptionpeer-revieweden_US
dc.description.abstractThe objective was to use ovulation synchronization with timed artificial insemination (TAI) to evaluate the effect of timing of artificial insemination (AI) with frozen sex-sorted sperm on fertility performance in pasture-based compact calving herds. Ejaculates from 3 Holstein-Friesian bulls were split and processed to provide frozen sex-sorted sperm (SS) at 4 × 106 sperm per straw, and frozen conventional sperm at 15 × 106 sperm per straw (CONV). A modified Progesterone-Ovsynch protocol was used for estrous synchronization, with TAI occurring 16 h after the second GnRH injection for cows assigned to CONV, and either 16 h (SS-16) or 22 h (SS-22) for cows assigned to SS. Pregnancy diagnosis was conducted by transrectal ultrasound scanning of the uterus 35 to 40 d after TAI (n = 2,175 records available for analysis). Generalized linear mixed models were used to examine the effects of treatment on pregnancy per artificial insemination (P/AI). Fixed effects included treatment (n = 3), bull (n = 3), treatment by bull interaction, parity (n = 4), days-in-milk category (n = 3), and treatment by days-in-milk category, with herd (n = 24) included as a random effect. Pregnancy per AI was greater for CONV compared with both SS-16 and SS-22 (61.1%, 49.0%, and 51.3%, respectively), and the SS treatments did not differ from each other (relative P/AI for SS-16 and SS-22 vs. CONV were 80.2% and 84.0%, respectively). There were significant bull and treatment by bull interaction effects. Additional analysis was undertaken using a model that included herd as a fixed effect. This analysis identified marked herd-to-herd variation (within-herd relative P/AI for the combined SS treatments vs. CONV ranged from 48–121%). The tertile of herds with the best performance achieved a mean relative P/AI of 100% (range = 91–121%), indicating that P/AI equivalent to CONV is achievable with SS. Conversely, the tertile of herds with the poorest performance achieved a mean relative P/AI of 67% (range = 48–77%). We found that SS resulted in poorer overall P/AI compared with CONV sperm regardless of timing of AI. Marked variation existed between herds; however, one-third of herds achieved P/AI results equal to CONV. Identification of factors responsible for the large herd-to-herd variation in P/AI with SS, and development of strategies to reduce this variation, warrant further research.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesJournal of Dairy Science;Vol 103
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectsex-sorted spermen_US
dc.subjectsynchronizationen_US
dc.subjectfixed time artificial inseminationen_US
dc.subjectseasonal calvingen_US
dc.titleEvaluation of delayed timing of artificial insemination with sex-sorted sperm on pregnancy per artificial insemination in seasonal-calving, pasture-based lactating dairy cowsen_US
dc.typeArticleen_US
dc.identifier.doihttps://doi.org/10.3168/jds.2020-18847
dc.contributor.sponsorIrish Dairy Levy Trusten_US
dc.contributor.sponsorIrish Department of Agriculture, Food and the Marineen_US
dc.contributor.sponsorGrantNumber15S732en_US
dc.source.volume103
dc.source.issue12
dc.source.beginpage12059
dc.source.endpage12068
refterms.dateFOA2023-12-19T15:28:22Z
dc.source.journaltitleJournal of Dairy Science


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