• Login
    View Item 
    •   T-Stór
    • Crops, Environment & Land Use Programme
    • Environment, Soils & Land Use
    • View Item
    •   T-Stór
    • Crops, Environment & Land Use Programme
    • Environment, Soils & Land Use
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of T-StórCommunitiesPublication DateAuthorsTitlesSubjectsFunderThis CollectionPublication DateAuthorsTitlesSubjectsFunderProfilesView

    My Account

    LoginRegister

    Information

    Deposit AgreementLicense

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    An inter-laboratory comparison of multi-enzyme and multiple substrate-induced respiration assays to assess method consistency in soil monitoring

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    An inter-laboratory comparison ...
    Size:
    193.5Kb
    Format:
    PDF
    Download
    Author
    Creamer, Rachel E.
    Bellamy, Pat
    Black, Helaina I. J.
    Cameron, Clare M.
    Campbell, Colin D.
    Chamberlain, Paul
    Harris, Jim
    Parekh, Nisha
    Pawlett, Mark
    Poskitt, Jan
    Stone, Dote
    Ritz, Karl
    Show allShow less
    Keyword
    CLPP
    Multiple substrate-induced respiration
    Repeatability
    Soil enzymes
    Soil monitoring
    Date
    01/07/2009
    
    Metadata
    Show full item record
    Statistics
    Display Item Statistics
    URI
    http://hdl.handle.net/11019/356
    Citation
    Rachel E. Creamer, Pat Bellamy, Helaina I. J. Black, Clare M. Cameron, Colin D. Campbell, Paul Chamberlain, Jim Harris, Nisha Parekh, Mark Pawlett, Jan Poskitt, Dote Stone, Karl Ritz. An inter-laboratory comparison of multi-enzyme and multiple substrate-induced respiration assays to assess method consistency in soil monitoring. Biology and Fertility of Soils, 2009, 45(6), pp 623-633. DOI: 10.1007/s00374-009-0374-y
    Abstract
    The use of indicators in soil monitoring schemes to detect changes in soil quality is receiving increased attention, particularly the application of soil biological methods. However, to date, the ability to compare information from different laboratories applying soil microbiological techniques in broad-scale monitoring has rarely been taken into account. This study aimed to assess the consistency and repeatability of two techniques that are being evaluated for use as microbiological indicators of soil quality; multi-enzyme activity assay and multiple substrate induced respiration (MSIR). Data was tested for intrinsic (within-plate) variation, inter-laboratory repeatability (geometric mean regression and correlation coefficient) and land-use discrimination (principal components analysis, PCA). Intrinsic variation was large for both assays suggesting that high replicate numbers will be required. Inter-laboratory repeatability showed diverging patterns for the enzyme assay and MSIR. Discrimination of soils was significant for both techniques with relatively consistent patterns, however combined laboratory discrimination analyses for each technique showed inconsistent correspondence between the laboratories. These issues could be addressed through the adoption of reliable analytical standards for biological methods along with adequate replication. However, until the former is addressed, dispersed analyses are not currently advisable for monitoring schemes.
    Funder
    UK Department of Environment, Food and Rural Affairs (Defra)
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1007/s00374-009-0374-y
    Scopus Count
    Collections
    Environment, Soils & Land Use
    Environment, Soils & Land Use
    Environment, Soils & Land Use

    entitlement

     
    DSpace software copyright © 2002-2017  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.