Show simple item record

dc.contributor.authorCreamer, Rachel E.*
dc.contributor.authorBellamy, Pat*
dc.contributor.authorBlack, Helaina I. J.*
dc.contributor.authorCameron, Clare M.*
dc.contributor.authorCampbell, Colin D.*
dc.contributor.authorChamberlain, Paul*
dc.contributor.authorHarris, Jim*
dc.contributor.authorParekh, Nisha*
dc.contributor.authorPawlett, Mark*
dc.contributor.authorPoskitt, Jan*
dc.contributor.authorStone, Dote*
dc.contributor.authorRitz, Karl*
dc.date.accessioned2013-05-08T15:12:12Z
dc.date.available2013-05-08T15:12:12Z
dc.date.issued2009-07
dc.identifier.citationCreamer, R., Bellamy, P., Black, H. J., Cameron, C., Campbell, C., Chamberlain, P., Harris, J., Parekh, N., Pawlett, M., Poskitt, J., Stone, D. & Ritz, K. 2009. An inter-laboratory comparison of multi-enzyme and multiple substrate-induced respiration assays to assess method consistency in soil monitoring. Biology and Fertility of Soils, 45, 623-633en_GB
dc.identifier.issn0178-2762
dc.identifier.urihttp://hdl.handle.net/11019/387
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe use of indicators in soil monitoring schemes to detect changes in soil quality is receiving increased attention, particularly the application of soil biological methods. However, to date, the ability to compare information from different laboratories applying soil microbiological techniques in broad-scale monitoring has rarely been taken into account. This study aimed to assess the consistency and repeatability of two techniques that are being evaluated for use as microbiological indicators of soil quality: multi-enzyme activity assay and multiple substrate-induced respiration (MSIR). Data were tested for intrinsic (within-assay plate) variation, inter-laboratory repeatability (geometric mean regression and correlation coefficient) and land-use discrimination (principal components analysis). Intrinsic variation was large for both assays suggesting that high replicate numbers are required. Inter-laboratory repeatability showed diverging patterns for the enzyme assay and MSIR. Discrimination of soils was significant for both techniques with relatively consistent patterns; however, combined laboratory discrimination analyses for each technique showed inconsistent correspondence between the laboratories. These issues could be addressed through the adoption of reliable analytical standards for biological methods along with adequate replication. However, until the former is addressed, dispersed analyses are not currently advisable for monitoring schemes.en_GB
dc.description.sponsorshipUK Department of Environment, Food and Rural Affairs (Defra)en_GB
dc.language.isoenen_GB
dc.publisherSpringer-Verlagen_GB
dc.relation.ispartofseriesBiology and Fertility of Soils;vol 45
dc.subjectCLPPen_GB
dc.subjectMultiple substrate-induced respirationen_GB
dc.subjectRepeatabilityen_GB
dc.subjectSoil enzymesen_GB
dc.subjectSoil monitoringen_GB
dc.titleAn inter-laboratory comparison of multi-enzyme and multiple substrate-induced respiration assays to assess method consistency in soil monitoringen_GB
dc.typeArticleen_GB
dc.identifier.rmisJCEV-0324-5879
dc.identifier.doihttp://dx.doi.org/10.1007/s00374-009-0374-y
dc.contributor.sponsorUK Department of Environment, Food and Rural Affairs (Defra)
refterms.dateFOA2018-01-12T07:51:00Z


Files in this item

Thumbnail
Name:
Interlaboratory Compasion BFS.pdf
Size:
193.5Kb
Format:
PDF

This item appears in the following Collection(s)

Show simple item record