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dc.contributor.authorFouhy, Fiona*
dc.contributor.authorGuinane, Caitriona M.*
dc.contributor.authorHussey, Seamus*
dc.contributor.authorWall, Rebecca*
dc.contributor.authorRyan, C. Anthony*
dc.contributor.authorDempsey, Eugene*
dc.contributor.authorMurphy, Brendan*
dc.contributor.authorRoss, R Paul*
dc.contributor.authorFitzgerald, Gerald F*
dc.contributor.authorSTANTON, CATHERINE*
dc.contributor.authorCotter, Paul D.*
dc.date.accessioned2013-08-23T15:35:07Z
dc.date.available2013-08-23T15:35:07Z
dc.date.issued04/09/2012
dc.identifier.citationFouhy, Fiona et al. High-Throughput Sequencing Reveals the Incomplete, Short-Term Recovery of Infant Gut Microbiota following Parenteral Antibiotic Treatment with Ampicillin and Gentamicin. Antimicrob. Agents Chemother., November 2012 vol. 56 no. 11: 5811-5820. doi: 10.1128/AAC.00789-12en_GB
dc.identifier.issn0066-4804
dc.identifier.urihttp://hdl.handle.net/11019/436
dc.descriptionpeer-revieweden_GB
dc.description.abstractThe infant gut microbiota undergoes dramatic changes during the first 2 years of life. The acquisition and development of this population can be influenced by numerous factors, and antibiotic treatment has been suggested as one of the most significant. Despite this, however, there have been relatively few studies which have investigated the short-term recovery of the infant gut microbiota following antibiotic treatment. The aim of this study was to use high-throughput sequencing (employing both 16S rRNA and rpoB-specific primers) and quantitative PCR to compare the gut microbiota of nine infants who underwent parenteral antibiotic treatment with ampicillin and gentamicin (within 48 h of birth), 4 and 8 weeks after the conclusion of treatment, relative to that of nine matched healthy controls. The investigation revealed that the gut microbiota of the antibiotic-treated infants had significantly higher proportions of Proteobacteria (P = 0.0049) and significantly lower proportions of Actinobacteria (P = 0.00001) (and the associated genus Bifidobacterium [P = 0.0132]) as well as the genus Lactobacillus (P = 0.0182) than the untreated controls 4 weeks after the cessation of treatment. By week 8, the Proteobacteria levels remained significantly higher in the treated infants (P = 0.0049), but the Actinobacteria, Bifidobacterium, and Lactobacillus levels had recovered and were similar to those in the control samples. Despite this recovery of total Bifidobacterium numbers, rpoB-targeted pyrosequencing revealed that the number of different Bifidobacterium species present in the antibiotic-treated infants was reduced. It is thus apparent that the combined use of ampicillin and gentamicin in early life can have significant effects on the evolution of the infant gut microbiota, the long-term health implications of which remain unknown.en_GB
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology EMBARK scholarship; Teagasc Walsh Fellowship; Science Foundation Ireland (Investigator award 11/PI/1137); Science Foundation of Ireland-funded Centre for Science, Engineering and Technology, the Alimentary Pharmabiotic Centre.en_GB
dc.language.isoenen_GB
dc.publisherAmerican Society for Microbiologyen_GB
dc.relation.ispartofseriesAntimicrobial Agents and Chemotherapy;vol 56
dc.subjectAntibioticsen_GB
dc.subjectInfanten_GB
dc.subjectGut microbiotaen_GB
dc.subjectHigh-throughput sequencingen_GB
dc.subjectAmpicillinen_GB
dc.subjectGentamycinen_GB
dc.subjectBifidobacteriumen_GB
dc.titleHigh-Throughput Sequencing Reveals the Incomplete, Short-Term Recovery of Infant Gut Microbiota following Parenteral Antibiotic Treatment with Ampicillin and Gentamicinen_GB
dc.typeArticleen_GB
dc.identifier.rmisMDBY-0106-5971
dc.identifier.doihttp://dx.doi.org/10.1128/AAC.00789-12
dc.contributor.sponsorIrish Research Council for Science, Engineering and Technology
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumberSFI/11/PI/1137
refterms.dateFOA2018-01-12T07:45:14Z


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