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dc.contributor.authorLiskova, Kamila*
dc.contributor.authorAuty, Mark*
dc.contributor.authorChaurin, Valerie*
dc.contributor.authorMin, Soyoung*
dc.contributor.authorMok, K. Hun*
dc.contributor.authorO'Brien, Nora M.*
dc.contributor.authorKelly, Alan L.*
dc.contributor.authorBrodkorb, Andre*
dc.date.accessioned2013-11-27T14:17:45Z
dc.date.available2013-11-27T14:17:45Z
dc.date.issued19/08/2011
dc.identifier.citationLišková, K., Auty, M. A. E., Chaurin, V., Min, S., Mok, K. H., O'Brien, N., Kelly, A. L. and Brodkorb, A. (2011), Cytotoxic complexes of sodium oleate with β-lactoglobulin. Eur. J. Lipid Sci. Technol., 113: 1207–1218. doi: 10.1002/ejlt.201100109en_GB
dc.identifier.issn1438-7697
dc.identifier.urihttp://hdl.handle.net/11019/453
dc.descriptionpre-printen_GB
dc.description.abstractA complex of α-lactalbumin and oleic acid has previously been shown to induce apoptosis in cancer cells in a number of in vitro and in vivo trials. This complex is called HAMLET or BAMLET, depending on the origin of α-la (human/bovine alpha-lactalbumin made lethal to tumour cells). In the current study, it was shown that bovine β-lactoglobulin (β-lg), upon binding sodium oleate (NaOle), the salt of oleic acid, also acquires cytotoxicity towards tumour cells (human monocytic cells U937), analogously to HAMLET/BAMLET complexes. The properties of the complex were characterized using FIR spectroscopy, HPLC and SDS-PAGE. It was shown that the level of covalent oligomerization (dimers and trimers) of β-lg increased with increasing the molar ratio of sodium oleate NaOle:β-lg in the preparation procedure. At the same time, increasing the molar ratio of NaOle:β-lg increased the cytotoxicity of the complex. The increase in cytotoxicity appeared to be dependent on the amount of bound NaOle in the complex, but not on the content of multimeric forms of β-lg. The NaOle/β-lg complex also showed similarity with BAMLET in penetrating the cell membrane and co-localizing with the cell nucleus. Furthermore, DNA fragmentation studies suggested that tumour cells (U937) treated with the complex died by apoptosis, as in the case of BAMLET, and healthy cells appeared to be less affected by treatment, as shown with model rat adrenal pheochromocytoma cells PC12. In conclusion, β-lg and NaOle can form complexes with apoptosis-inducing qualities comparable to those of BAMLET.en_GB
dc.description.sponsorshipThe work was funded by the Irish Dairy Research Trust and The Department of Agriculture (Food Institutional Research Measure – FIRM project 08RDTMFRC650) under the National Development Plan 2007-2013. K. Lišková was funded under the TeagascWalsh Fellowship Scheme.
dc.language.isoenen_GB
dc.publisherWiley VCH-Verlag GmbH & Co.en_GB
dc.relation.ispartofseriesEuropean Journal of Lipid Science and Technology;vol 113
dc.subjectβ-lactoglobulinen_GB
dc.subjectOleic acid/oleateen_GB
dc.subjectHAMLET/BAMLETen_GB
dc.subjectDenaturation and aggregationen_GB
dc.subjectCytotoxicityen_GB
dc.subjectApoptosisen_GB
dc.titleCytotoxic Complexes of Sodium Oleate with β-Lactoglobulinen_GB
dc.typeArticleen_GB
dc.identifier.rmisMDDT-0103-5947
dc.identifier.doihttp://dx.doi.org/10.1002/ejlt.201100109
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine
dc.contributor.sponsorTeagasc Walsh Fellowship Programme
dc.contributor.sponsorIrish Dairy Levy Research Trust
dc.contributor.sponsorGrantNumber08RDTMFRC650
refterms.dateFOA2018-01-12T07:47:44Z


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