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dc.contributor.authorKeegan, Jemma*
dc.contributor.authorO'Kennedy, Richard*
dc.contributor.authorCrooks, Steven*
dc.contributor.authorElliott, C.*
dc.contributor.authorBrandon, David*
dc.contributor.authorDanaher, Martin*
dc.date.accessioned2014-01-02T16:07:38Z
dc.date.available2014-01-02T16:07:38Z
dc.date.issued14/01/2011
dc.identifier.citationJemma Keegan, Richard O’Kennedy, Steven Crooks, Christopher Elliott, David Brandon, Martin Danaher. Detection of benzimidazole carbamates and amino metabolites in liver by surface plasmon resonance-biosensor. Analytica Chimica Acta, 2011, 700 (1–2), 41–48. DOI: 10.1016/j.aca.2010.12.041en_GB
dc.identifier.issn0003-2670
dc.identifier.urihttp://hdl.handle.net/11019/482
dc.descriptionThis research was funded by the Irish Department of Agriculture, Fisheries and Food under the Food Institutional Research Measure as part of the National Development Plan (Project 05/R&D/TN/355)
dc.descriptionpeer-revieweden_GB
dc.description.abstractTwo surface plasmon resonance (SPR) biosensor screening assays were developed and validated to detect 11 benzimidazole carbamate (BZT) and four amino-benzimidazole veterinary drug residues in liver tissue. The assays used polyclonal antibodies, raised in sheep, to detect BZTs and amino-benzimidazoles. A modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction method was developed to isolate benzimidazole carbamate residues. Liver samples were extracted using an acetonitrile extraction method. BZTs were purified by dispersive solid phase extraction (d-SPE) using C18 sorbent. Residues of amino-benzimidazoles were effectively cleaned-up using a simple cyclohexane defatting step. The assays were validated in accordance with the performance criteria described in 2002/657/EC. The BZT assay limit of detection was calculated to be 32 μg kg−1, the detection capability (CCβ) was determined to be 50 μg kg−1 and the mean recovery of analytes was in the range 77–132%. The amino-benzimidazole assay limit of detection was determined to be 41 μg kg−1, the CCβ was determined to be 75 μg kg−1 and analyte recovery was in the range 103–116%. Biosensor assay performance was tested by analysing liver tissue from animals treated with benzimidazole drugs and comparing the results with an ultra high performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) confirmatory method. All non-compliant samples were identified using the biosensor assays.en_GB
dc.description.sponsorshipDepartment of Agriculture, Food and the Marineen_GB
dc.language.isoenen_GB
dc.publisherElsevieren_GB
dc.relation.ispartofseriesAnalytica Chimica Acta;vol 700
dc.subjectSPR biosensoren_GB
dc.subjectBenzimidazolesen_GB
dc.subjectLiveren_GB
dc.subjectScreening assayen_GB
dc.titleDetection of benzimidazole carbamates and amino metabolites in liver by surface plasmon resonance-biosensoren_GB
dc.typeArticleen_GB
dc.identifier.doihttp://dx.doi.org/10.1016/j.aca.2010.12.041
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine, Ireland
dc.contributor.sponsorGrantNumber05/R&D/TN/355
refterms.dateFOA2019-06-14T07:10:26Z


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