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    β-lactoglobulin as a molecular carrier of linoleate: characterisation and effects on intestinal epithelial cells in vitro

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    Author
    Le Maux, Solene
    Giblin, Linda
    Croguennec, Thomas
    Bouhallab, Said
    Brodkorb, Andre cc
    Keyword
    β-lactoglobulin
    Sodium Linoleate/Linoleic Acid
    Stoichiometry
    Caco-2
    Cytotoxicity
    Date
    27/08/2012
    
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    URI
    http://hdl.handle.net/11019/506
    Citation
    Le Maux, S., Giblin, L., Croguennec, T., Bouhallab, S., & Brodkorb, A. (2012). β-lactoglobulin as a molecular carrier of linoleate: characterisation and effects on intestinal epithelial cells in vitro. Journal of Agricultural and Food Chemistry, 60(37), 9476–9483. doi: 10.1021/jf3028396
    Abstract
    The dairy protein β-lactoglobulin (βlg) is known to bind hydrophobic ligands such as fatty acids. In the present work, we investigated the biological activity in vitro of linoleate once complexed to bovine βlg. Binding of linoleate (C18:2) to bovine βlg was achieved by heating at 60 °C for 30 min at pH 7.4, resulting in a linoleate/βlg molar binding stoichiometry of 1.1, 2.1, and 3.4. Two types of binding sites were determined by ITC titrations. Binding of linoleate induced the formation of covalent dimers and trimers of βlg. The LD50 on Caco-2 cells after 24 h was 58 μM linoleate. However, cell viability was unaffected when 200 μM linoleate was presented to the Caco-2 cells as part of the βlg complex. The Caco-2 cells did not increase mRNA transcript levels of long chain fatty acid transport genes, FATP4 and FABPpm, or increase levels of the cAMP signal, in response to the presence of 50 μM linoleate alone or as part of the βlg complex. Therefore, it is proposed that βlg can act as a molecular carrier and alter the bioaccessibility of linoleate/linoleic acid.
    Funder
    Department of Agriculture, Food and the Marine; Irish Research Council for Science, Engineering and Technology; Teagasc Walsh Fellowship Programme
    Grant Number
    08/RD/TMFRC/650
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1021/jf3028396
    Scopus Count
    Collections
    Food Biosciences
    Food Chemistry & Technology
    Food Chemistry & Technology
    Food Chemistry & Technology

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