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    New Insights into Cell Encapsulation and the Role of Proteins During Flow Cytometry

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    No 1828 Doherty 2012 Open Access ...
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    Author
    Doherty, Sinead B.
    Brodkorb, Andre cc
    Keyword
    Cell Encapsulation
    Proteins
    Flow Cytometry
    Fluorescence-Activated Cell Sorting
    High throughput screening
    Process Analytical Technology
    Bioprocessing
    Cytometric assays
    Date
    13/06/2012
    
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    URI
    http://hdl.handle.net/11019/567
    Citation
    Sinead B. Doherty and A. Brodkorb (2012). New Insights into Cell Encapsulation and the Role of Proteins During Flow Cytometry, Flow Cytometry - Recent Perspectives, M.Sc. Ingrid Schmid (Ed.), ISBN: 978-953-51-0626-5, InTech, DOI: 10.5772/48039
    Abstract
    Modern approaches to science tend to follow divergent paths. On one hand, instruments and technologies are developed to capture as much information as possible with the need for complex data analysis to identify problematic issues. On the other hand, formulation focused, minimalistic approaches that gather only the most pertinent data for specific questions also represent a powerful methodology. This chapter will provide many examples of the latter by integrating Flow Cytometry (FACS - Fluorescence-Activated Cell Sorting) technology with high throughput screening (HTS) of encapsulation systems with extensive utility of one-dimensional (1-D) imaging for protein localisation. In this regard, less information is acquired from each cell, data files will be more manageable, easier to analyse and throughput screening will be significantly enhanced beyond traditional HTS analysis, irrespective of the protein concentration present in the background or delivery media.
    Funder
    Irish National Development Plan 2007 to 2013; Irish Dairy Levy Research Trust; Science Foundation Ireland
    Grant Number
    NU518
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.5772/48039
    Scopus Count
    Collections
    Food Chemistry & Technology
    Food Chemistry & Technology

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