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dc.contributor.authorDoherty, Sinead B.*
dc.contributor.authorBrodkorb, Andre*
dc.date.accessioned2014-03-25T15:08:07Z
dc.date.available2014-03-25T15:08:07Z
dc.date.issued13/06/2012
dc.identifier.citationSinead B. Doherty and A. Brodkorb (2012). New Insights into Cell Encapsulation and the Role of Proteins During Flow Cytometry, Flow Cytometry - Recent Perspectives, M.Sc. Ingrid Schmid (Ed.), ISBN: 978-953-51-0626-5, InTech, DOI: 10.5772/48039en_GB
dc.identifier.isbn9.78954E+12
dc.identifier.urihttp://hdl.handle.net/11019/567
dc.descriptionpeer-revieweden_GB
dc.description.abstractModern approaches to science tend to follow divergent paths. On one hand, instruments and technologies are developed to capture as much information as possible with the need for complex data analysis to identify problematic issues. On the other hand, formulation focused, minimalistic approaches that gather only the most pertinent data for specific questions also represent a powerful methodology. This chapter will provide many examples of the latter by integrating Flow Cytometry (FACS - Fluorescence-Activated Cell Sorting) technology with high throughput screening (HTS) of encapsulation systems with extensive utility of one-dimensional (1-D) imaging for protein localisation. In this regard, less information is acquired from each cell, data files will be more manageable, easier to analyse and throughput screening will be significantly enhanced beyond traditional HTS analysis, irrespective of the protein concentration present in the background or delivery media.en_GB
dc.description.sponsorshipSome of the cytometric work presented in this chapter was supported by the Irish Dairy Research Trust project NU518 “Probiotic Protection”, the Irish National Development Plan 2007 to 2013 and Science Foundation Ireland (SFI).
dc.language.isoenen_GB
dc.publisherIntechen_GB
dc.subjectCell Encapsulationen_GB
dc.subjectProteinsen_GB
dc.subjectFlow Cytometryen_GB
dc.subjectFluorescence-Activated Cell Sortingen_GB
dc.subjectHigh throughput screeningen_GB
dc.subjectProcess Analytical Technologyen_GB
dc.subjectBioprocessingen_GB
dc.subjectCytometric assaysen_GB
dc.titleNew Insights into Cell Encapsulation and the Role of Proteins During Flow Cytometryen_GB
dc.typeBook chapteren_GB
dc.identifier.rmisMDDB-0106-5457
dc.identifier.rmisMDBY-0106-6277
dc.identifier.doihttp://dx.doi.org/10.5772/48039
dc.contributor.sponsorIrish National Development Plan 2007 to 2013
dc.contributor.sponsorIrish Dairy Levy Research Trust
dc.contributor.sponsorScience Foundation Ireland
dc.contributor.sponsorGrantNumberNU518
refterms.dateFOA2018-01-12T07:29:02Z


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