A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to β-casomorphin
dc.contributor.author | Keating, A.F. | * |
dc.contributor.author | Smith, T.J. | * |
dc.contributor.author | Ross, R Paul | * |
dc.contributor.author | Cairns, M.T. | * |
dc.date.accessioned | 2014-07-24T11:52:58Z | |
dc.date.available | 2014-07-24T11:52:58Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | A.F. Keating, T.J. Smith, R.P. Ross and M.T. Cairns. A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to β-casomorphin. Irish Journal of Agricultural and Food Research 47: 99–104, 2008 | en_GB |
dc.identifier.issn | 0791-6833 | |
dc.identifier.uri | http://hdl.handle.net/11019/632 | |
dc.description | peer-reviewed | en_GB |
dc.description | This work was supported by Enterprise Ireland and by a Teagasc Walsh fellowship to A.F. Keating. | |
dc.description.abstract | Two genetic variants of the bovine β-casein gene (A1 and B) encode a histidine residue at codon 67, resulting in potential liberation of a bioactive peptide, β-casomorphin, upon digestion. An allele-specific PCR (AS-PCR) was evaluated to distinguish between the β-casomorphin-releasing variants (A1 and B) and the non-releasing variants. AS-PCR successfully distinguished β-casein variants in 41 of 42 animals as confirmed by sequence analysis. Overall, while the incidence of the homozygous A1 and B animals (i.e., homozygous for the histidine residue; 21.4%) was lower than that for animals without the histidine residue (30.9% respectively), 69% of animals carried at least one allele for the histidine residue at codon 67. | en_GB |
dc.description.sponsorship | Teagasc Walsh Fellowship Programme | en_GB |
dc.description.sponsorship | Enterprise Ireland | |
dc.language | English | |
dc.language.iso | en | en_GB |
dc.publisher | Teagasc, Oak Park, Carlow, Ireland | en_GB |
dc.relation.ispartofseries | Irish Journal of Agricultural and Food Research;vol 47 | |
dc.subject | Allele-specific PCR | en_GB |
dc.subject | β-casein | en_GB |
dc.subject | Bioactive peptides | en_GB |
dc.title | A note on the evaluation of a beta-casein variant in bovine breeds by allele-specific PCR and relevance to β-casomorphin | en_GB |
dc.type | Article | en_GB |
refterms.dateFOA | 2018-01-12T07:25:34Z |
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IJAFR volume 47, 2008 [18]
Irish Journal of Agricultural and Food Research, volume 47, 2008