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    Novel High-Molecular Weight Fucosylated Milk Oligosaccharides Identified in Dairy Streams

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    Author
    Mehra, Raj
    Barile, Daniela
    Marotta, Mariarosaria
    Lebrilla, Carlitto B.
    Chu, Caroline
    German, J.Bruce
    Keyword
    Bovine Milk Oligosaccharides
    Dairy streams
    Infant milk formula
    Breast milk
    Date
    08/05/2014
    
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    URI
    http://hdl.handle.net/11019/718
    Citation
    Mehra R, Barile D, Marotta M, Lebrilla CB, Chu C, et al. (2014) Novel High-Molecular Weight Fucosylated Milk Oligosaccharides Identified in Dairy Streams. PLoS ONE 9(5): e96040. doi:10.1371/journal.pone.0096040
    Abstract
    Oligosaccharides are the third largest component in human milk. This abundance is remarkable because oligosaccharides are not digestible by the newborn, and yet they have been conserved and amplified during evolution. In addition to encouraging the growth of a protective microbiota dominated by bifidobacteria, oligosaccharides have anti-infective activity, preventing pathogens from binding to intestinal cells. Although it would be advantageous adding these valuable molecules to infant milk formula, the technologies to reproduce the variety and complexity of human milk oligosaccharides by enzymatic/organic synthesis are not yet mature. Consequently, there is an enormous interest in alternative sources of these valuable oligosaccharides. Recent research has demonstrated that bovine milk and whey permeate also contain oligosaccharides. Thus, a thorough characterization of oligosaccharides in bovine dairy streams is an important step towards fully assessing their specific functionalities. In this study, bovine milk oligosaccharides (BMOs) were concentrated by membrane filtration from a readily available dairy stream called “mother liquor”, and analyzed by high accuracy MALDI FT-ICR mass spectrometry. The combination of HPLC and accurate mass spectrometry allowed the identification of ideal processing conditions leading to the production of Kg amount of BMO enriched powders. Among the BMOs identified, 18 have high-molecular weight and corresponded in size to the most abundant oligosaccharides present in human milk. Notably 6 oligosaccharides contained fucose, a sugar monomer that is highly abundant in human milk, but is rarely observed in bovine milk. This work shows that dairy streams represent a potential source of complex milk oligosaccharides for commercial development of unique dairy ingredients in functional foods that reproduce the benefits of human milk.
    Funder
    University of California Discovery Program; National Institute of Environmental Health Sciences; National Institutes of Health; California Dairy Research Foundation; CHARGE study; Department of Agriculture, Food and the Marine
    Grant Number
    05GEB01NHB; P42ES004699; R01AT00707; 08 GEB-04 NH; P01 ES11269; FIRM–05/R&D/TD/368
    ae974a485f413a2113503eed53cd6c53
    http://dx.doi.org/10.1371/journal.pone.0096040
    Scopus Count
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    Food Biosciences
    Food Biosciences
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