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dc.contributor.authorMehra, Raj*
dc.contributor.authorBarile, Daniela*
dc.contributor.authorMarotta, Mariarosaria*
dc.contributor.authorLebrilla, Carlitto B.*
dc.contributor.authorChu, Caroline*
dc.contributor.authorGerman, J.Bruce*
dc.date.accessioned2014-09-03T11:20:20Z
dc.date.available2014-09-03T11:20:20Z
dc.date.issued08/05/2014
dc.identifier.citationMehra R, Barile D, Marotta M, Lebrilla CB, Chu C, et al. (2014) Novel High-Molecular Weight Fucosylated Milk Oligosaccharides Identified in Dairy Streams. PLoS ONE 9(5): e96040. doi:10.1371/journal.pone.0096040en_GB
dc.identifier.urihttp://hdl.handle.net/11019/718
dc.descriptionpeer-revieweden_GB
dc.description.abstractOligosaccharides are the third largest component in human milk. This abundance is remarkable because oligosaccharides are not digestible by the newborn, and yet they have been conserved and amplified during evolution. In addition to encouraging the growth of a protective microbiota dominated by bifidobacteria, oligosaccharides have anti-infective activity, preventing pathogens from binding to intestinal cells. Although it would be advantageous adding these valuable molecules to infant milk formula, the technologies to reproduce the variety and complexity of human milk oligosaccharides by enzymatic/organic synthesis are not yet mature. Consequently, there is an enormous interest in alternative sources of these valuable oligosaccharides. Recent research has demonstrated that bovine milk and whey permeate also contain oligosaccharides. Thus, a thorough characterization of oligosaccharides in bovine dairy streams is an important step towards fully assessing their specific functionalities. In this study, bovine milk oligosaccharides (BMOs) were concentrated by membrane filtration from a readily available dairy stream called “mother liquor”, and analyzed by high accuracy MALDI FT-ICR mass spectrometry. The combination of HPLC and accurate mass spectrometry allowed the identification of ideal processing conditions leading to the production of Kg amount of BMO enriched powders. Among the BMOs identified, 18 have high-molecular weight and corresponded in size to the most abundant oligosaccharides present in human milk. Notably 6 oligosaccharides contained fucose, a sugar monomer that is highly abundant in human milk, but is rarely observed in bovine milk. This work shows that dairy streams represent a potential source of complex milk oligosaccharides for commercial development of unique dairy ingredients in functional foods that reproduce the benefits of human milk.en_GB
dc.description.sponsorshipThis project was supported by the University of California Discovery Program (05GEB01NHB), the National Institute of Environmental Health Sciences (P42ES004699), the National Institutes of Health award R01AT00707, the California Dairy Research Foundation (08 GEB-04 NH) and the CHARGE study (P01 ES11269). The authors acknowledge financial support from the Irish Department of Agriculture, Fisheries and Food through the Food Institutional Research Measure (FIRM–05/R&D/TD/368).
dc.language.isoenen_GB
dc.publisherPLOSen_GB
dc.relation.ispartofseriesPLOS ONE;vol 9
dc.subjectBovine Milk Oligosaccharidesen_GB
dc.subjectDairy streamsen_GB
dc.subjectInfant milk formulaen_GB
dc.subjectBreast milken_GB
dc.titleNovel High-Molecular Weight Fucosylated Milk Oligosaccharides Identified in Dairy Streamsen_GB
dc.typeArticleen_GB
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0096040
dc.contributor.sponsorUniversity of California Discovery Program
dc.contributor.sponsorNational Institute of Environmental Health Sciences
dc.contributor.sponsorNational Institutes of Health
dc.contributor.sponsorCalifornia Dairy Research Foundation
dc.contributor.sponsorCHARGE study
dc.contributor.sponsorDepartment of Agriculture, Food and the Marine
dc.contributor.sponsorGrantNumber05GEB01NHB
dc.contributor.sponsorGrantNumberP42ES004699
dc.contributor.sponsorGrantNumberR01AT00707
dc.contributor.sponsorGrantNumber08 GEB-04 NH
dc.contributor.sponsorGrantNumberP01 ES11269
dc.contributor.sponsorGrantNumberFIRM–05/R&D/TD/368
refterms.dateFOA2018-01-12T08:07:51Z


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